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Series GSE142024 Query DataSets for GSE142024
Status Public on May 06, 2020
Title The RNA helicase DDX5 supports mitochondrial function in small cell lung cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary DDX5 is a founding member of the DEAD-box RNA helicase family, which are a group of enzymes that regulate ribonucleoprotein (RNP) formation and function in every aspect of RNA metabolism. Our lab previously found that DDX5 is involved in energy homeostasis, a process altered in many cancers. Small cell lung cancer (SCLC) is an understudied cancer type that lacks effective treatment. Therefore, we investigated the roles of DDX5 in SCLC. We show here that DDX5 is overexpressed in SCLC cell lines. Depletion of DDX5 results in reduced growth and mitochondrial dysfunction in the chemoresistant SCLC cell line H69AR. The latter is evidenced by downregulation of genes involved in oxidative phosphorylation and impaired oxygen consumption,. Interestingly, depletion of DDX5 specifically leads to reduction of intracellular succinate, a TCA intermediate that serves as a direct electron donor to mitochondrial complex II. Taken together, we propose that the oncogenic role of DDX5 is at least in part manifested through upregulation of respiration to support the energy demands of cancer cells. This also suggests that defects in RNP remodeling are connected to energy metabolism and carcinogenesis.
Overall design Six samples
Contributor(s) Xing Z, Russon MP, Utturkar SM, Tran EJ
Citation(s) 32376686
Submission date Dec 13, 2019
Last update date Aug 05, 2020
Contact name Elizabeth J Tran
Phone 7654963889
Organization name Purdue University
Department Biochemistry
Lab Tran
Street address 175 S. University Street
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4217775 WT rep1
GSM4217776 WT rep2
GSM4217777 WT rep3
BioProject PRJNA595593
SRA SRP237546

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142024_raw_counts.txt.gz 478.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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