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| Status |
Public on May 06, 2020 |
| Title |
The RNA helicase DDX5 supports mitochondrial function in small cell lung cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DDX5 is a founding member of the DEAD-box RNA helicase family, which are a group of enzymes that regulate ribonucleoprotein (RNP) formation and function in every aspect of RNA metabolism. Our lab previously found that DDX5 is involved in energy homeostasis, a process altered in many cancers. Small cell lung cancer (SCLC) is an understudied cancer type that lacks effective treatment. Therefore, we investigated the roles of DDX5 in SCLC. We show here that DDX5 is overexpressed in SCLC cell lines. Depletion of DDX5 results in reduced growth and mitochondrial dysfunction in the chemoresistant SCLC cell line H69AR. The latter is evidenced by downregulation of genes involved in oxidative phosphorylation and impaired oxygen consumption,. Interestingly, depletion of DDX5 specifically leads to reduction of intracellular succinate, a TCA intermediate that serves as a direct electron donor to mitochondrial complex II. Taken together, we propose that the oncogenic role of DDX5 is at least in part manifested through upregulation of respiration to support the energy demands of cancer cells. This also suggests that defects in RNP remodeling are connected to energy metabolism and carcinogenesis.
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| Overall design |
Six samples
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| Contributor(s) |
Xing Z, Russon MP, Utturkar SM, Tran EJ |
| Citation(s) |
32376686 |
| Submission date |
Dec 13, 2019 |
| Last update date |
Aug 05, 2020 |
| Contact name |
Elizabeth J Tran |
| E-mail(s) |
ejtran@purdue.edu
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| Phone |
7654963889
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| Organization name |
Purdue University
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| Department |
Biochemistry
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| Lab |
Tran
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| Street address |
175 S. University Street
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| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA595593 |
| SRA |
SRP237546 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142024_raw_counts.txt.gz |
478.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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