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| Status |
Public on Dec 01, 2021 |
| Title |
mRNA expression profiles (transcriptome) of human endothelial cells (cell line: TIME) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the transcriptome of human endothelial cells (cell line: TIME) after supplementation with polyunsaturated fatty acids (PUFA) and/or stimulation with pro-inflammatory cytokines. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling 144 h. Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of the cytokines IL-1β, TNF-α, and IFN-γ each in a concentration of 5 ng/ml.
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| Overall design |
mRNA profiles of human endothelial cells (cell line: TIME) supplemented with unsaturated fatty acids and exposed to an inflammatory milieu were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.
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| Contributor(s) |
Schumann J, Roessler C |
| Citation(s) |
34831066, 37301912 |
| Submission date |
Dec 12, 2019 |
| Last update date |
Sep 08, 2023 |
| Contact name |
Julia Schumann |
| E-mail(s) |
julia.schumann@uk-halle.de
|
| Organization name |
University Medicine Halle (Saale)
|
| Street address |
Franzosenweg 1a
|
| City |
Halle (Saale) |
| ZIP/Postal code |
06112 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE142090 |
mRNA expression profiles (transcriptome) of TIME and RAW264.7 |
|
| Relations |
| BioProject |
PRJNA595385 |
| SRA |
SRP237441 |
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