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Series GSE141957 Query DataSets for GSE141957
Status Public on Dec 01, 2021
Title mRNA expression profiles (transcriptome) of human endothelial cells (cell line: TIME)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report the transcriptome of human endothelial cells (cell line: TIME) after supplementation with polyunsaturated fatty acids (PUFA) and/or stimulation with pro-inflammatory cytokines. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling 144 h. Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of the cytokines IL-1β, TNF-α, and IFN-γ each in a concentration of 5 ng/ml.
Overall design mRNA profiles of human endothelial cells (cell line: TIME) supplemented with unsaturated fatty acids and exposed to an inflammatory milieu were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.
Contributor(s) Schumann J, Roessler C
Citation(s) 34831066, 37301912
Submission date Dec 12, 2019
Last update date Sep 08, 2023
Contact name Julia Schumann
Organization name University Medicine Halle (Saale)
Street address Franzosenweg 1a
City Halle (Saale)
ZIP/Postal code 06112
Country Germany
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM4217056 TIME6
GSM4217057 TIME9
GSM4217058 TIME98
This SubSeries is part of SuperSeries:
GSE142090 mRNA expression profiles (transcriptome) of TIME and RAW264.7
BioProject PRJNA595385
SRA SRP237441

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141957_TIME.counts.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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