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| Status |
Public on Jan 23, 2020 |
| Title |
Single cell RNA sequencing of Hic1 and PDGFRa-labeled cells in the heart |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The cardiac stroma contains multipotent mesenchymal progenitors. However, lineage relationships within cardiac stromal cells are poorly defined. Here, we identify heart-resident PDGFRa+ SCA-1+ cells as cardiac Fibro/Adipogenic Progenitors (cFAPs) and show that they respond to ischemic damage by generating SCA-1- fibrogenic cells. Pharmacological blockade of this differentiation step with an anti-fibrotic tyrosine kinase inhibitor decreases post-myocardial infarction (MI) remodeling and leads to improvement in heart function.
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| Overall design |
To understand the heterogeneity of cardiac mesenchymal progenitors in the heart, hearts were digested and cell suspensions of Hic1 CreERT2 tdTomato+, Hic1-citrine+ and PDGFRa H2BGFP+ cells were subjected to single cell RNA sequencing. Hic1 CreERT2 tdTomato+ and PDGFRa H2BGFP+ cells were collected from undamaged mice as well as 7 days after left anterior descending (LAD) coronary artery ligation. Hic1 citrine+ cells were only collected from undamaged mice. For the Hic1 CreERT2 tdTomato samples (for each time point), 3 mice hearts were pooled together in one sample. For the PDGFRa H2BGFP mice 4 mice hearts were hashed with anti-MHC I antibodies and pooled (2 undamaged and 2 7 days after LAD ligation). For the Hic1 citrine, cells from 3 undamaged hearts were hashed and pooled together and sequenced as 1 sample.
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| Contributor(s) |
Soliman H, Vander Werff R |
| Citation(s) |
32142665 |
| |
| Submission date |
Dec 12, 2019 |
| Last update date |
Apr 23, 2020 |
| Contact name |
Fabio Rossi |
| E-mail(s) |
hesham@brc.ubc.ca
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| Phone |
6042219496
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| Organization name |
University of British Columbia
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| Department |
School of Biomedical Engineering
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| Lab |
Rossi Lab
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| Street address |
2222 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| GSM4216418 |
Hic1 CreERT2 tdTomato+ cells_undamaged |
| GSM4216419 |
Hic1 CreERT2 tdTomato+ cells_7d post LAD ligation |
| GSM4216420 |
Hic1 citrine+ cells_undamaged |
| GSM4216421 |
PDGFRa H2BGFP+ cells_undamaged+7d_post_LAD ligation |
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| This SubSeries is part of SuperSeries: |
| GSE141929 |
Heart-resident PDGFRa+ SCA-1+ cells |
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| Relations |
| BioProject |
PRJNA595321 |
| SRA |
SRP237348 |
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