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Series GSE141785 Query DataSets for GSE141785
Status Public on Mar 21, 2023
Title Characterization of a Distinct Rectal Mucosal Transcriptome among Transgender Women utilizing Cross-Sex Hormone Therapy
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary A comparison of rectal mucosal RNA transcriptome findings between transgender women using feminizing hormone therapy, men who have sex with men engaging in receptive anal intercourse, and males who had never engaged in anal intercourse demonstrates differential gene expression involving pathways critical for mucosal inflammation, suggesting the urgent need for further exploration into the immunologic effects of cross-sex hormone therapy in the rectal mucosa and the potential impact on HIV transmission risk at this site.
Overall design Rectal mucosal biopsies were obtained from 9 transgender women using cross-sex hormone therapy and from these, total RNA was extracted for RNA-Seq analysis. The RM transcriptome of TGW was compared to stored specimens from a prior study that included 10 MSM engaging in CRAI and 10 men who reported no history of AI; the previously stored specimens were re-sequenced from extracted RNA to control for batch effect.
Contributor(s) Ackerley CG, Billingsley JM, Tharp G, Amancha PK, Smith SA, Amara RR, Bosinger S, Kelley CF
Citation(s) 32611248
Submission date Dec 10, 2019
Last update date Mar 21, 2023
Contact name Gregory K Tharp
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (29)
GSM4213203 1_1-CFAR_14-83527-001
GSM4213204 2_2-CFAR_14-83527-002
GSM4213205 3_3-CFAR_14-83527-003
BioProject PRJNA594726
SRA SRP235589

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Supplementary file Size Download File type/resource
GSE141785_Kelley_normalized_readcounts.txt.gz 5.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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