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Series GSE141658 Query DataSets for GSE141658
Status Public on May 31, 2020
Title An extension to: Systematic assessment of commercially available low-input miRNA library preparation kits
Organisms Homo sapiens; synthetic construct
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary We compared the performance of two commercial kits (NEBNext and NEXTflex) for miRNA detection sensitivity, reliability, titration response and ability to detect differentially expressed miRNAs at different amounts of synthetic miRNAs. We observed differences in detection sensitivity between the kits, but none were able to detect the full repertoire of expected miRNAs. The reliability within the replicates of all kits was good, while larger differences were observed between the kits, although none could accurately quantify the majority of miRNAs. In a previous publication (; GEO accession number GSE133719) we accessed the performance of six additional miRNA library preparation kits.
Overall design Systematic comparison of miRNA profiles assessed in low-input synthetic miRNA samples and samples from pools of blood derived CD8 T cells of rheumatoid arthritis (RA) patients or healthy controls (HC) by six commercially available library preparation kits. In a previous publication (; GEO accession number GSE133719) we accessed the performance of six additional miRNA library preparation kits.
Contributor(s) Heinicke F, Gilfillan GD
Citation(s) 32436772
Submission date Dec 09, 2019
Last update date Nov 02, 2020
Contact name Fatima Heinicke
Organization name Oslo University Hospital
Department Department of Medical Genetics
Street address Kirkeveien 166
City Oslo
ZIP/Postal code 0450
Country Norway
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL19604 Illumina HiSeq 2500 (synthetic construct)
Samples (42)
GSM4210663 NEBNext A 1
GSM4210664 NEBNext A 2
GSM4210665 NEBNext A 3
BioProject PRJNA594317
SRA SRP235240

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Supplementary file Size Download File type/resource
GSE141658_Composer.biologic.matureRef.fasta.gz 28.5 Kb (ftp)(http) FASTA
GSE141658_Composer.synthetic.matureRef.fasta.gz 11.0 Kb (ftp)(http) FASTA
GSE141658_countTable_biological.csv.gz 21.5 Kb (ftp)(http) CSV
GSE141658_countTable_biological_downsampled.csv.gz 13.8 Kb (ftp)(http) CSV
GSE141658_countTable_biological_rlog_downsampled.csv.gz 80.0 Kb (ftp)(http) CSV
GSE141658_countTable_synthetic.csv.gz 67.7 Kb (ftp)(http) CSV
GSE141658_countTable_synthetic_downsampled.csv.gz 54.7 Kb (ftp)(http) CSV
GSE141658_countTable_synthetic_rlog_downsampled.csv.gz 157.5 Kb (ftp)(http) CSV
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Processed data are available on Series record

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