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| Status |
Public on Sep 14, 2021 |
| Title |
CD4+ T cells contribute to neurodegeneration in Lewy body dementia [CSF_and_PBMCs_Healthy and LBD] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Recent studies indicate that the adaptive immune system plays a role in Lewy body dementia (LBD). However, the mechanism regulating T cell brain homing in LBD is unknown. Here, we observed T cells adjacent to Lewy bodies and dopaminergic neurons in post-mortem LBD brains. Single-cell RNA sequencing of cerebrospinal fluid (CSF) identified upregulated expression of C-X-C Motif Chemokine Receptor 4 (CXCR4) in CD4+ T cells in LBD. CSF protein levels of the CXCR4 ligand, C-X-C Motif Chemokine Ligand 12 (CXCL12) were associated with neuroaxonal damage in LBD. Furthermore, we observed clonal expansion and upregulated Interleukin 17A expression by CD4+ T cells stimulated with a phosphorylated α-synuclein epitope. Thus, CXCR4-CXCL12 signaling may represent a mechanistic target for inhibiting pathological interleukin-17-producing T cell trafficking in LBD.
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| Overall design |
scRNAseq of CSF cells from healthy (n=11) and LBD (n=11) subjects. Live cells were sorted with a live/dead SYTOX blue dye. Sequencing libraries were prepared using the 10X Genomics platform.
PBMCs from some of these subjects were also sequenced by scRNAseq.
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| Contributor(s) |
Gate D, Leventhal O, Fehlmann T |
| Citation(s) |
34648304 |
| Submission date |
Dec 06, 2019 |
| Last update date |
Dec 14, 2021 |
| Contact name |
David Gate |
| E-mail(s) |
dgate@northwestern.edu
|
| Phone |
8054044775
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| Organization name |
Northwestern University
|
| Street address |
303 E Chicago Ave
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL28038 |
DNBSEQ-G400 (Homo sapiens) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA593998 |
| SRA |
SRP234979 |
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