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Series GSE141578 Query DataSets for GSE141578
Status Public on Sep 14, 2021
Title CD4+ T cells contribute to neurodegeneration in Lewy body dementia [CSF_and_PBMCs_Healthy and LBD]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Recent studies indicate that the adaptive immune system plays a role in Lewy body dementia (LBD). However, the mechanism regulating T cell brain homing in LBD is unknown. Here, we observed T cells adjacent to Lewy bodies and dopaminergic neurons in post-mortem LBD brains. Single-cell RNA sequencing of cerebrospinal fluid (CSF) identified upregulated expression of C-X-C Motif Chemokine Receptor 4 (CXCR4) in CD4+ T cells in LBD. CSF protein levels of the CXCR4 ligand, C-X-C Motif Chemokine Ligand 12 (CXCL12) were associated with neuroaxonal damage in LBD. Furthermore, we observed clonal expansion and upregulated Interleukin 17A expression by CD4+ T cells stimulated with a phosphorylated α-synuclein epitope. Thus, CXCR4-CXCL12 signaling may represent a mechanistic target for inhibiting pathological interleukin-17-producing T cell trafficking in LBD.
Overall design scRNAseq of CSF cells from healthy (n=11) and LBD (n=11) subjects. Live cells were sorted with a live/dead SYTOX blue dye. Sequencing libraries were prepared using the 10X Genomics platform.

PBMCs from some of these subjects were also sequenced by scRNAseq.
Contributor(s) Gate D, Leventhal O, Fehlmann T
Citation(s) 34648304
Submission date Dec 06, 2019
Last update date Dec 14, 2021
Contact name David Gate
Phone 8054044775
Organization name Northwestern University
Street address 303 E Chicago Ave
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
Platforms (2)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL28038 DNBSEQ-G400 (Homo sapiens)
Samples (35)
GSM4208766 CSF14_PD
GSM4208768 CSF10_PD
GSM4208769 CSF21_PD
BioProject PRJNA593998
SRA SRP234979

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141578_RAW.tar 277.9 Mb (http)(custom) TAR (of MTX, TAR, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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