|
| Status |
Public on Oct 19, 2022 |
| Title |
Gene regulation by PCGF1 in hematopoietic progenitor cells [ChIP, MNaseSeq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Polycomb proteins are epigenetic regulators important for cell fate decision and maintenance. Here we show that PCGF1, one of components of Polycomb repressive complex (PRC) 1 is important for the differentiation of hematopoietic stem/progenitor cells (HSPCs) towards lymphoid lineage. The deletion of Pcgf1 in HSPCs lead to severe defects in B cell development with an expansion of myeloid progenitors. The blockade of B cell differentiation occurred at the lymphoid-primed multipotent progenitor (LMPP) stage. Chromatin immunoprecipitation followed by DNA sequencing analysis of normal LMPP-like cells demonstrated that PCGF1 colocalized with RING1A/B and PRC2. Upon deletion of Pcgf1, we found dramatic decline of H3K27me3 levels, resulting in de-repression of target genes. Strikingly, the occupancy of RING1A/B, PRC2 and monoubiquitination of histone H2A at lysin 119 on the target loci was not affected. These results demonstrate that the PCGF1 safeguards the immune system development by directly regulating the PRC2-mediated H3K27me3 levels.
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| |
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| Overall design |
ChIP, MNaseSeq
|
| |
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| Contributor(s) |
Takano J, Ikawa T, Koseki H |
| Citation missing |
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| |
| Submission date |
Dec 06, 2019 |
| Last update date |
Oct 22, 2022 |
| Contact name |
Junichiro Takano |
| E-mail(s) |
junichiro.takano@riken.jp
|
| Organization name |
RIKEN
|
| Department |
IMS
|
| Lab |
Developmental Genetics
|
| Street address |
Tsurumi-ku-Suehirocho-1-7-22
|
| City |
Yokohama-shi |
| State/province |
Kanagawa-kwn |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platforms (2) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (44)
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| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA593952 |
| SRA |
SRP234926 |
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