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Series GSE141559 Query DataSets for GSE141559
Status Public on Oct 19, 2022
Title Gene regulation by PCGF1 in hematopoietic progenitor cells [ChIP, MNaseSeq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb proteins are epigenetic regulators important for cell fate decision and maintenance. Here we show that PCGF1, one of components of Polycomb repressive complex (PRC) 1 is important for the differentiation of hematopoietic stem/progenitor cells (HSPCs) towards lymphoid lineage. The deletion of Pcgf1 in HSPCs lead to severe defects in B cell development with an expansion of myeloid progenitors. The blockade of B cell differentiation occurred at the lymphoid-primed multipotent progenitor (LMPP) stage. Chromatin immunoprecipitation followed by DNA sequencing analysis of normal LMPP-like cells demonstrated that PCGF1 colocalized with RING1A/B and PRC2. Upon deletion of Pcgf1, we found dramatic decline of H3K27me3 levels, resulting in de-repression of target genes. Strikingly, the occupancy of RING1A/B, PRC2 and monoubiquitination of histone H2A at lysin 119 on the target loci was not affected. These results demonstrate that the PCGF1 safeguards the immune system development by directly regulating the PRC2-mediated H3K27me3 levels.
Overall design ChIP, MNaseSeq
Contributor(s) Takano J, Ikawa T, Koseki H
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Submission date Dec 06, 2019
Last update date Oct 22, 2022
Contact name Junichiro Takano
Organization name RIKEN
Department IMS
Lab Developmental Genetics
Street address Tsurumi-ku-Suehirocho-1-7-22
City Yokohama-shi
State/province Kanagawa-kwn
ZIP/Postal code 230-0045
Country Japan
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (44)
GSM4206934 Pcgf1_f/f_input_1
GSM4206935 Pcgf1_f/f_input_2
GSM4206936 Pcgf1_-/-_input 1
This SubSeries is part of SuperSeries:
GSE141560 Hematopoietic progenitor cells
BioProject PRJNA593952
SRA SRP234926

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141559_H3K27me3_Peaks.txt.gz 3.3 Mb (ftp)(http) TXT
GSE141559_Pcgf1_Peak.broadPeak.gz 61.0 Kb (ftp)(http) BROADPEAK
GSE141559_RAW.tar 2.3 Gb (http)(custom) TAR (of BW)
GSE141559_Ring1b_Peak.broadPeak.gz 602.2 Kb (ftp)(http) BROADPEAK
GSE141559_samples_withpeakfiles.txt.gz 232 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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