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Series GSE141487 Query DataSets for GSE141487
Status Public on May 31, 2021
Title Variant PCGF1-PRC1 links PRC2 recruitment with induced transcriptional inactivation at target genes [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Polycomb repressive complexes-1 and -2 (PRC1 and 2), silence developmental genes in spatiotemporally regulated manner during metazoan embryogenesis. How PcG proteins contribute to down-regulation of target genes upon differentiation, however, remains elusive. Here, by differentiating embryonic stem cells into embryoid bodies, we reveal a crucial role of a PCGF1 (Polycomb group RING finger protein 1)-containing variant PRC1 complex (PCGF1-PRC1) to facilitate induced down-regulation of a group of genes. Binding of PCGF1-PRC1 results in down-regulation of transcriptional activity, followed by Histone H2AK119 mono-ubiquitination (H2AK119ub1) and subsequent recruitment of PRC2, to initiate PcG-repressive domain formation. Based on these findings, we propose that PCGF1-PRC1 mediates establishment of PcG-repressive domains at target genes during differentiation.
Overall design RNA-seq analysis of mouse ES cells and embryoid body (EB)
Contributor(s) Sugishita H, Koseki H
Citation(s) 34504070
Submission date Dec 04, 2019
Last update date Sep 29, 2021
Contact name Hiroki Sugishita
Organization name RIKEN
Department IMS
Lab Laboratory for Developmental Genetics
Street address 1-7-22, Suehiro-cho
City Tsurumi-ku,Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (59)
GSM4204603 ERT2-Cre_Ring1aKO_Ring1bControl_Control_ES_rep1
GSM4204604 ERT2-Cre_Ring1aKO_Ring1bControl_Mutant_ES_rep1
GSM4204605 ERT2-Cre_Ring1aKO_Ring1bControl_Control_EB_rep1
This SubSeries is part of SuperSeries:
GSE141488 Variant PCGF1-PRC1 links PRC2 recruitment with induced transcriptional inactivation at target genes
BioProject PRJNA593601
SRA SRP234740

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141487_RAW.tar 14.4 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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