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Series GSE141216 Query DataSets for GSE141216
Status Public on Oct 29, 2021
Title RNA-seq analysis of mRNA profiles in the livers of Mettl3 flox/flox and hepatocyte-specific Mettl3 knockout (HKO) mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study is to compare transcriptome profilings of liver from Mettl3 flox/flox and hepatocyte-specific Mettl3 knockout (HKO) mice.
Methods: Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from livers of Mettl3 flox/flox and HKO mice at 8 weeks old. mRNA profiles were generated by deep sequencing using an Illumina HiSeq X Ten platform. Paired-end clean reads were aligned to the mouse reference genome(Ensemble_GRCm38.90) with Hisat2 (version 2.0.4), and the aligned reads were used to quantify mRNA expression by using HTSeq (version 0.9.1).
Conclusion: The hepatic mRNA profiles in Mettl3 flox/flox and HKO mice were characterized.
Overall design RNA samples were pooled from livers of METTL3 HKO and Mettl3 flox/flox mice (n=4 for each group). mRNA profiles of HKO and Mettl3 flox/flox livers were generated by deep sequencing, in a single test, using Illumina HiSeq X Ten platform.
Contributor(s) Chen Z, Li X
Citation(s) 34893641, 35927268
Submission date Nov 30, 2019
Last update date Aug 16, 2022
Contact name Zheng Chen
Phone +8645186402029
Organization name Harbin Institute of Technology
Street address 92 Xidazhijie Harbin Institute of Technology
City Harbin
State/province Heilongjiang
ZIP/Postal code 150001
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (2)
GSM4197416 flox
GSM4197417 HKO
This SubSeries is part of SuperSeries:
GSE141325 RNA-seq and ATAC-seq of liver samples in Mettl3 flox/flox and hepatocyte-specific Mettl3 knockout (HKO) mice
BioProject PRJNA592704
SRA SRP234074

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Supplementary file Size Download File type/resource
GSE141216_RAW.tar 1.9 Mb (http)(custom) TAR (of XLSX)
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Raw data are available in SRA
Processed data are available on Series record

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