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Series GSE141189 Query DataSets for GSE141189
Status Public on Nov 25, 2021
Title Depicting human endothelial to hematopoietic transition and the effects of modulating pyruvate catabolism on blood formation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Hematopoietic stem cell (HSC) quiescence, maintenance and differentiation are closely linked to changes in metabolism. However, the role of metabolism during the first emergence of HSCs from hemogenic endothelium (HE) is still unknown. In this study, we focus on the implication of energetic metabolic pathways during endothelial to hematopoietic transition (EHT). EHT is accompanied by a metabolic switch, with concomitant increases in glycolysis and oxidative phosphorylation (OXPHOS). Inhibiting glycolysis in HE cells leads to a block in hematopoietic lineage specification. An OXPHOS fuel, glutamine, is essential for HSC emergence and provides distinct intermediates to mediate differentiation towards the two major hematopoietic lineages, GPA+ and CD45+ cells. Strikingly, shifting the fate of pyruvate from glycolysis to the TCA cycle redirects HE specification towards CD45+ cells at the expense of GPA+ cells and vice versa. As such, our findings reveal the plasticity of hematopoietic lineage specification during EHT which can be modulated by metabolism.
Overall design Transcriptomic analysis of hiPSC-derived HE, EHT, HSC-like cells and cord blood CD34+ cells. Comparison of control HE cells with UK5099- or DCA-treated HE cells.

***Raw data not provided due to patient privacy concerns***
Contributor(s) Mansell E, Canals I, Guibentif C, Soneji S, Woods N, Oburoglu L
Citation(s) 34914165
Submission date Nov 29, 2019
Last update date Feb 18, 2022
Contact name Shamit Soneji
Organization name BMC
Street address Solvegatan 19
City Lund
ZIP/Postal code 22184
Country Sweden
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (7)
GSM4196707 hiPSC-derived HE cells
GSM4196708 hiPSC-derived EHT cells
GSM4196709 hiPSC-derived HSC-like cells
BioProject PRJNA592550

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141189_RAW.tar 90.7 Mb (http)(custom) TAR (of TXT)
Raw data not provided for this record
Processed data provided as supplementary file

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