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Series GSE141092 Query DataSets for GSE141092
Status Public on Nov 28, 2019
Title miRNA array experiment of NC-Exo and GATA4-Exo
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Exosomes carry cell-specific proteins or mRNAs/miRNAs that mediate the functional effect of exosomes. To investigate whether miRNAs within GATA4-Exo are important for their cardioprotective effects, we utilized an unbiased approach by performing an exosomal miRNA array analysis.
Total RNA was extracted from CDCs-Exo by using a Qiagen miRNeasy Mini Kit. Global expression patterns of miRNAs were then examined by using Affymetrix GeneChip miRNA 4.0 arrays.Expression data were generated by using Affymetrix Expression Console software and normalized according to the MAS5 method. The RVM t test was applied to filter the differentially expressed genes for the control and experimental groups. Differentially expressed genes were defined based on a P-value threshold and fold-change analysis.
Overall design Exosomes were obtained from the CDC and CDCGATA4 culture supernatants by ultracentrifugation. Exosomes were harvested from CDCs by ultracentrifugation. miRNA arrays were performed to test the differential miRNA expression between exosomes derived from GATA4-overexpressing CDCs (GATA4-Exo) and control CDCs (NC-Exo). Microarray analysis revealed significantly increased and decreased miRNAs expression in GATA4-Exo.
Contributor(s) Hao C, Chen L
Citation(s) 32586406
Submission date Nov 27, 2019
Last update date Jul 07, 2020
Contact name chunshu Hao
Phone 15850511967
Organization name Southeast university
Department Cardiology
Street address No.87 Dingjiaqiao street
City Nanjing
ZIP/Postal code 210009
Country China
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (6)
GSM4194916 NC-Exo, rep1
GSM4194917 NC-Exo, rep2
GSM4194918 NC-Exo, rep3
BioProject PRJNA592052

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141092_RAW.tar 4.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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