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Series GSE141076 Query DataSets for GSE141076
Status Public on Feb 06, 2020
Title m6A MeRIP-Seq of interscapular brown adipose tissue (iBAT) in Mettl3flox/flox and BAT-specific Mettl3 knockout (BKO) mice
Organism Mus musculus
Experiment type Other
Summary Purpose: Through mRNA m6A-profiling, we aim to characterize the mRNA m6A changes in iBAT obtained from Mettl3flox/flox and BKO mice.
Methods: Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from iBAT of Mettl3 flox/flox and BKO mice at 8 weeks old. Each sample (300 μg total RNA) was pooled from 5 mice for each group. Poly(A)+ RNA was purified using Dynabeads™ mRNA Purification Kit (Invitrogen) following the manufacturer’s instructions. Chemically fragmented poly(A)+ RNA was incubated with m6A antibody (Synaptic System, 202003) for immunoprecipitation following the standard protocol of Magna MeRIPTM m6A Kit (MERK, 17-10499). Enrichment of m6A mRNA was then analyzed by high-throughput sequencing using Illumina Hiseq X platform. The m6A peaks were detected by MACS2. The motif search was detected by HOMER.
Conclusion: The iBAT mRNA m6A profiles in Mettl3flox/flox and BKO mice were characterized.
 
Overall design To map the mRNA m6A modification caused by METTL3 in iBAT, MeRIP-seq was performed in iBAT obtained from Mettl3flox/flox and BKO mice.
 
Contributor(s) Chen Z, Wang Y
Citation(s) 32245957
Submission date Nov 26, 2019
Last update date Apr 13, 2020
Contact name Zheng Chen
E-mail(s) chenzheng@hit.edu.cn
Phone +8645186402029
Organization name Harbin Institute of Technology
Street address 92 Xidazhijie Harbin Institute of Technology
City Harbin
State/province Heilongjiang
ZIP/Postal code 150001
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM4194548 flox_IP
GSM4194549 BKO_IP
GSM4194550 flox_input
Relations
BioProject PRJNA591942
SRA SRP233375

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Supplementary file Size Download File type/resource
GSE141076_peaks_comparable.xlsx 3.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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