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Series GSE141067 Query DataSets for GSE141067
Status Public on Jun 11, 2020
Title Dynamic regulation of histone modifications and long-range chromosomal interactions during post-mitotic transcriptional reactivation [Hi-C]
Organism Homo sapiens
Experiment type Other
Summary During mitosis, transcription of genomic DNA is dramatically reduced, before its reactivation occurs during nuclear reformation in anaphase/telophase. Many aspects of the underlying principles that mediate transcriptional memory and reactivation in the daughter cells remain unclear. Here, we used ChIP-seq on synchronized cells at different stages after mitosis to generate genome-wide maps of histone modifications. In combination with EU-RNA-seq and Hi-C analyses, we show that, during prometaphase, promoters, enhancers, and insulators retain H3K4me3 and H3K4me1, while losing H3K27ac. Enhancers harboring mitotic H3K4me1 are associated with cell type-specific transcription factor motifs and mitotic activation of cell identity genes. As cells exit mitosis, promoters regain H3K27ac, which correlates with transcriptional reactivation. Insulators also gain H3K27ac and CCCTC binding factors (CTCF) in anaphase/telophase. This increase may play a role in the establishment of topologically associating domains (TADs). Together, our results show that the genome is reorganized in sequential order, in which histone methylations occur first in prometaphase, histone acetylation and CTCF in anaphase/telophase, transcription in cytokinesis, and long-range chromatin interactions in early G1. Our results provide insights into the histone modification landscape that allows faithful reestablishment of the transcriptional program and TADs during cell division.
Overall design Hi-C during mitosis-G1 in U2OS cells
Contributor(s) Kang H, Xu Z, Dixon JR, Hetzer MW
Citation(s) 32499403
Submission date Nov 26, 2019
Last update date Mar 28, 2022
Contact name April Elizabeth Williams
Phone 7345461645
Organization name Salk Institute for Biological Studies
Department IGC
Street address 10010 N Torrey Pines Rd
City San Diego
State/province California
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (16)
GSM4194449 0 min-Hi-C-r1
GSM4194450 0 min-Hi-C-r2
GSM4194451 35 min-Hi-C-r1
This SubSeries is part of SuperSeries:
GSE141139 Dynamic regulation of histone modifications and long-range chromosomal interactions during post-mitotic transcriptional reactivation
BioProject PRJNA591920
SRA SRP233368

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Supplementary file Size Download File type/resource
GSE141067_RAW.tar 18.2 Gb (http)(custom) TAR (of HIC)
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Raw data are available in SRA
Processed data provided as supplementary file

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