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Series GSE141053 Query DataSets for GSE141053
Status Public on Nov 27, 2019
Title Effect of metformin on histone modifications in lung cancer cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The DNA regions that were associated with the metformin altered expressions of H3K4me3 and/or H3K27me3 were investigated by ChIP-seq, and the regions significantly up- or down-regulated by metformin were identified in H1299 cells. Over 80% of H3K4me3 were discovered within promoter regions, but less than 10% of H3K27me3 were observed in this area. Metformin significantly downregulated H3K4me3 level at the promoter regions of 1113 genes, but upregulated levels in the promoter regions of 1492 genes. Gene enrichment analysis revealed that abundant cell cycle related genes such as PIK3C3, CCNB2, CDK1, CDK6, and E2F8 were upregulated, while certain cell cycle suppressor genes such as CDKN1A (p21) and DDIT4 were downregulated. No specific biological functional cluster was detected in the H3K27me3 up- or downregulated genes.
Overall design Examination of H3K4me3 and H3K27me3 in H1299 cells treated with or without 5mM metformin.
Contributor(s) Kim D, Kim DH
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Submission date Nov 26, 2019
Last update date Nov 29, 2019
Contact name Dongho Kim
Phone 82312996440
Organization name Sungkyunkwan University School of Medicine
Department Samsung Biomedical Research Institute
Lab Molecular Cell Biology
Street address #300 Chunchun-dong, Jangan-Ku, Suwon, Kyungggi-do
City Suwon
ZIP/Postal code 440-746
Country South Korea
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4193228 Con-H3K4me3
GSM4193229 Con-H3K27me3
GSM4193230 Con-Input
This SubSeries is part of SuperSeries:
GSE141060 Effect of metformin on mRNA expression, histone modifications, and chromatin accessibility in lung cancer cells
BioProject PRJNA591893
SRA SRP233305

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Supplementary file Size Download File type/resource
GSE141053_RAW.tar 5.0 Mb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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