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| Status |
Public on Nov 27, 2019 |
| Title |
Effect of metformin on mRNA expression in lung cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The genes whose transcription was significantly altered by metformin were investigated in H1299 cells by RNA-seq. Significantly up- or down-regulated genes were identified by cutoff value of |FC| >= 1.5. Metformin significantly downregulated mRNA levels of 499 genes while upregulating 615. KEGG (Kyoto Encyclopedia of Genes and Genomes) gene enrichment analysis revealed that the highly ranked clusters were cell cycle, apoptosis, cellular senescence and p53 signaling pathways. Multiple cell cycle checkpoint genes such as CCNA, CCNB, CCNE, CDK1, E2F2, E2F6, and E2F8 were downregulated, while certain cell cycle inhibitors such as CDKN2B (p15), CDKN1A (p21), DDIT4, and GADD45 were upregulated.
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| Overall design |
Examination of the transcriptome in H1299 cells treated with or without 5mM metformin.
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| Contributor(s) |
Kim D, Kim DH |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 26, 2019 |
| Last update date |
Nov 29, 2019 |
| Contact name |
Dongho Kim |
| E-mail(s) |
jindonghao2001@hotmail.com
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| Phone |
82312996440
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| Organization name |
Sungkyunkwan University School of Medicine
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| Department |
Samsung Biomedical Research Institute
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| Lab |
Molecular Cell Biology
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| Street address |
#300 Chunchun-dong, Jangan-Ku, Suwon, Kyungggi-do
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| City |
Suwon |
| ZIP/Postal code |
440-746 |
| Country |
South Korea |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE141060 |
Effect of metformin on mRNA expression, histone modifications, and chromatin accessibility in lung cancer cells |
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| Relations |
| BioProject |
PRJNA591888 |
| SRA |
SRP233304 |
