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Series GSE141052 Query DataSets for GSE141052
Status Public on Nov 27, 2019
Title Effect of metformin on mRNA expression in lung cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The genes whose transcription was significantly altered by metformin were investigated in H1299 cells by RNA-seq. Significantly up- or down-regulated genes were identified by cutoff value of |FC| >= 1.5. Metformin significantly downregulated mRNA levels of 499 genes while upregulating 615. KEGG (Kyoto Encyclopedia of Genes and Genomes) gene enrichment analysis revealed that the highly ranked clusters were cell cycle, apoptosis, cellular senescence and p53 signaling pathways. Multiple cell cycle checkpoint genes such as CCNA, CCNB, CCNE, CDK1, E2F2, E2F6, and E2F8 were downregulated, while certain cell cycle inhibitors such as CDKN2B (p15), CDKN1A (p21), DDIT4, and GADD45 were upregulated.
Overall design Examination of the transcriptome in H1299 cells treated with or without 5mM metformin.
Contributor(s) Kim D, Kim DH
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Submission date Nov 26, 2019
Last update date Nov 29, 2019
Contact name Dongho Kim
Phone 82312996440
Organization name Sungkyunkwan University School of Medicine
Department Samsung Biomedical Research Institute
Lab Molecular Cell Biology
Street address #300 Chunchun-dong, Jangan-Ku, Suwon, Kyungggi-do
City Suwon
ZIP/Postal code 440-746
Country South Korea
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM4193226 Control [RNA-seq]
GSM4193227 Metformin [RNA-seq]
This SubSeries is part of SuperSeries:
GSE141060 Effect of metformin on mRNA expression, histone modifications, and chromatin accessibility in lung cancer cells
BioProject PRJNA591888
SRA SRP233304

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Supplementary file Size Download File type/resource
GSE141052_RAW.tar 1.2 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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