GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE140961 Query DataSets for GSE140961
Status Public on Mar 25, 2020
Title RNA sequencing analysis of ARV825 target genes in senescent cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Although cellular senescence acts primarily as a tumor suppression mechanism, the accumulation of senescent cells in vivo eventually exerts deleterious side effects through inflammatory/tumor-promoting factor secretion. Thus, the development of new drugs that cause the specific elimination of senescent cells, termed senolysis, is anticipated. Here, by an unbiased high-throughput screening of chemical compounds and a bio-functional analysis, we identify ARV825 as a promising senolytic drug. ARV825 treatment eliminates senescent hepatic stellate cells in obese mouse livers, accompanied by the reduction of liver cancer development. Furthermore, the elimination of chemotherapy-induced senescent cells by ARV825 increases the efficacy of chemotherapy against xenograft tumors in immunocompromised mice. These results reveal the vulnerability of senescent cells and open up possibilities for its control.
Overall design We performed an RNA sequencing (RNA-seq) analysis to identify the genes whose expression is downregulated by the ARV825 treatment in senescent human diploid fibroblasts )HDFs.
Doxorubicin-induced senescent HDFs were treated with or without ARV825 (10 nM) for 4 days and were subjected to the RNAseq analysis.
Contributor(s) Wakita M, Hara E
Citation(s) 32321921
Submission date Nov 25, 2019
Last update date May 08, 2020
Contact name Daisuke Okuzaki
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (4)
GSM4191448 TIG-3 cells_vehicle_4d_DXR1
GSM4191449 TIG-3 cells_10 nM ARV82_4d_DXR1
GSM4191450 TIG-3 cells_vehicle_4d_DXR2
BioProject PRJNA591563
SRA SRP233194

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140961_RAW.tar 560.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap