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| Status |
Public on May 08, 2020 |
| Title |
Transcriptomics profiling of the developmental progression of Trypanosoma brucei |
| Organism |
Trypanosoma brucei |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mitochondrial metabolic remodeling is a hallmark of the Trypanosoma brucei digenetic life cycle since the insect stage utilizes the cost-effective oxidative phosphorylation to generate ATP, while bloodstream cells switch to less energetically efficient aerobic glycolysis. Due to difficulties in acquiring enough parasites from the tsetse fly vector for biochemical analysis, the dynamics of the parasite´s mitochondrial metabolic rewiring in the vector have remained obscure. Here, we took advantage of in vitro-induced differentiation to follow changes at the RNA levels.
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| Overall design |
To determine global changes to the transcriptome across the in vitro induced developmental progression of RBP6OE 123 cells, we applied RNA-seq measurements and analyses
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| Contributor(s) |
Zikova A, Butter F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 21, 2019 |
| Last update date |
May 10, 2020 |
| Contact name |
Falk Butter |
| E-mail(s) |
f.butter@imb-mainz.de
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| Organization name |
Institute of Molecular Biology gGmbH
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| Department |
Quantitative Proteomics
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| Lab |
Falk Butter
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| Street address |
Ackermannweg 4
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| City |
Mainz |
| State/province |
Germany |
| ZIP/Postal code |
55128 |
| Country |
Germany |
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| Platforms (1) |
| GPL20572 |
Illumina NextSeq 500 (Trypanosoma brucei) |
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| Samples (24)
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| GSM4185422 |
PCF cells, 0d_4 |
| GSM4185423 |
PCF cells, 2d_1 |
| GSM4185424 |
PCF cells, 2d_2 |
| GSM4185425 |
PCF cells, 2d_3 |
| GSM4185426 |
PCF cells, 2d_4 |
| GSM4185427 |
PCF cells, 3d_1 |
| GSM4185428 |
PCF cells, 3d_2 |
| GSM4185429 |
PCF cells, 3d_3 |
| GSM4185430 |
PCF cells, 3d_4 |
| GSM4185431 |
PCF cells, 4d_1 |
| GSM4185432 |
PCF cells, 4d_2 |
| GSM4185433 |
PCF cells, 4d_3 |
| GSM4185434 |
PCF cells, 4d_4 |
| GSM4185435 |
PCF cells, 6d_1 |
| GSM4185436 |
PCF cells, 6d_2 |
| GSM4185437 |
PCF cells, 6d_3 |
| GSM4185438 |
PCF cells, 6d_4 |
| GSM4185439 |
PCF cells, 8d_1 |
| GSM4185440 |
PCF cells, 8d_2 |
| GSM4185441 |
PCF cells, 8d_3 |
| GSM4185442 |
PCF cells, 8d_4 |
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| Relations |
| BioProject |
PRJNA590981 |
| SRA |
SRP231162 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE140796_RAW.tar |
1.2 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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