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| Status |
Public on Oct 22, 2020 |
| Title |
Senescence induced by BMI1 inhibition is a therapeutic vulnerability in H3K27M-mutant DIPG |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
We studied the effect of BMI1 inhibition in diffuse intrinsic pontine glioma cells (DIPG).
We reported the application of chromatin immunoprecipitation sequencing for high-thoughput profiling of BMI1 and H2AK119ub in diffuse intrinsic pontine glioma cells (DIPG) in response to BMI1 inhibition.
We also performed bulk RNA sequencing of DIPG cells in response to pharmacological inhibition or knockdown of BMI1.
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| Overall design |
ChIP-seq samples were obtained using anti-BMI1 and anti-H2AK119ub antibodies in SU-DIPG04 and SF8628 DIPG cells with or without the BMI1 inhibitior, PTC-028.
RNA-seq samples were obtained from SU-DIPG04 and SF86828 DIPG cells treated with or without PTC-028 and from SU-DIPG04 and SF7761 DIPG cells expressing control shRNA or BMI1 shRNAs.
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| Contributor(s) |
Balakrishnan I, Danis E, Vibhakar R, Venkataraman S |
| Citation(s) |
33086074 |
| Submission date |
Nov 21, 2019 |
| Last update date |
Oct 22, 2020 |
| Contact name |
Etienne Danis |
| E-mail(s) |
etienne.danis@cuanschutz.edu
|
| Organization name |
University of Colorado, Denver - Anschutz Medical Campus
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| Street address |
12800 E. 19th Ave
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL27644 |
Illumina Novaseq 6000 (Homo sapiens) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA590880 |
| SRA |
SRP231142 |
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