GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE140768 Query DataSets for GSE140768
Status Public on Oct 22, 2020
Title Senescence induced by BMI1 inhibition is a therapeutic vulnerability in H3K27M-mutant DIPG
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary We studied the effect of BMI1 inhibition in diffuse intrinsic pontine glioma cells (DIPG).
We reported the application of chromatin immunoprecipitation sequencing for high-thoughput profiling of BMI1 and H2AK119ub in diffuse intrinsic pontine glioma cells (DIPG) in response to BMI1 inhibition.
We also performed bulk RNA sequencing of DIPG cells in response to pharmacological inhibition or knockdown of BMI1.

Overall design ChIP-seq samples were obtained using anti-BMI1 and anti-H2AK119ub antibodies in SU-DIPG04 and SF8628 DIPG cells with or without the BMI1 inhibitior, PTC-028.
RNA-seq samples were obtained from SU-DIPG04 and SF86828 DIPG cells treated with or without PTC-028 and from SU-DIPG04 and SF7761 DIPG cells expressing control shRNA or BMI1 shRNAs.
Contributor(s) Balakrishnan I, Danis E, Vibhakar R, Venkataraman S
Citation(s) 33086074
Submission date Nov 21, 2019
Last update date Oct 22, 2020
Contact name Etienne Danis
Organization name University of Colorado, Denver - Anschutz Medical Campus
Street address 12800 E. 19th Ave
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL27644 Illumina Novaseq 6000 (Homo sapiens)
Samples (30)
GSM4183376 input_SU-DIPG04_DMSO
GSM4183378 H2AK119_ChIP_SU-DIPG04_DMSO
BioProject PRJNA590880
SRA SRP231142

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140768_DIPG4_PTC028_raw_counts.txt.gz 279.2 Kb (ftp)(http) TXT
GSE140768_DIPG4_SF7761_BMI1_knockdown_raw_counts.txt.gz 293.0 Kb (ftp)(http) TXT
GSE140768_RAW.tar 3.8 Gb (http)(custom) TAR (of TDF)
GSE140768_SF8628_PTC028_raw_counts.txt.gz 278.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap