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Series GSE140396 Query DataSets for GSE140396
Status Public on May 11, 2021
Title Next Generation Sequencing Quantitative Analysis of TEAD Palmitoylation Inhibitor MGH-CP1 in Breast Cancer Cell Line
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study is to understand the signaling pathway alteration in cancer cell line treated with TEAD palmitoylation inhibitor MGH-CP1, and to further validate TEAD inhibitor for specifity in TEAD-YAP interuption.
Methods:Breast cancer cell line MDA-MB-231 was chosen to be treated with TEAD palmitoylation inhibitor MGH-CP1 at 10μM for 24 hours. Total RNA was isolated for the analysis. RNA samples were sent to Novogen for library construction, RNA sequencing and raw data process.
Results: MGH-CP1 specifically blocks TEAD transcriptional activity compared with YAP/TAZ siRNA in MDA-MB-231 cells.
Conclusions: Our study privides gene expression profiling evidence to validate our TEAD palmitoylation inhibitor MGH-CP1 as specific small molecule to block TEAD transcriptional activity.
We report the application of next generation sequencing technology for high-throughput profiling of TEAD palmitoylation inhibitor MGH-CP1 in breast cancer cells.
Overall design Examination of vehicle control and MGH-CP1 in one breast cancer cell line.
*** Submitter has indicated raw files are no longer available.
Contributor(s) Wu X, Sun Y
Citation(s) 32259481
Submission date Nov 14, 2019
Last update date May 11, 2021
Contact name Yang Sun
Phone 6177248603
Organization name Massachusetts General Hospital
Department CBRC
Street address 149 13th Street, CBRC 3050E
City Charlestown
State/province MA
ZIP/Postal code 02129
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM4160620 MDA-MB-231 cells, Control_1
GSM4160621 MDA-MB-231 cells, Control_2
GSM4160622 MDA-MB-231 cells, MGH-CP1-1
BioProject PRJNA589597

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Supplementary file Size Download File type/resource
GSE140396_processed_data-fpkm.txt.gz 1.9 Mb (ftp)(http) TXT
Raw data not provided for this record
Processed data are available on Series record

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