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Series GSE140341 Query DataSets for GSE140341
Status Public on Jan 06, 2021
Title Profiling of melocular mechanism of SOX17 effect during hematopoieisis of human ES cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Here, using SOX17-knockout and SOX17-inducible human PSCs, paired with cell biology and molecular profiling studies, we revealed that SOX17 represents a critical upstream factor that is required for activation and linkage of HOXA and arterial programs in hemogenic endothelium and establishing definitive lympho-myeloid hematopoiesis. These SOX17 effects are mediated through activation of NOTCH, CDX2 and retinoic acid signaling. Collectively, these findings are important to design strategies for direct HSC fate programming from hPSCs.
Overall design For ATAC-seq, DNA profiles of overexpression of SOX17 and non-overexpressed SOX17 were prepared in duplicate. For ChIP-seq, SOX17 and IgG antibodies in PHE. For RNA-seq, mRNA profiles of overexpression of SOX17 and non-overexpressed SOX17 were generated by sequencing, in triplicate.
Contributor(s) Jung HS, Liu P, Ong IM, Slukvin II
Citation(s) 33596423
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HL142665 Molecular Determinants of Hemogenic Endothelium UNIVERSITY OF WISCONSIN MADISON Igor I. Slukvin
U01 HL134655 Transplantation of MHC Homozygous Vascular Progenitors in Primates MORGRIDGE INSTITUTE FOR RESEARCH James Alexander Thomson
P51 OD011106 Wisconsin National Primate Research Center Support UNIVERSITY OF WISCONSIN MADISON MARSHA RUTH MAILICK
Submission date Nov 13, 2019
Last update date Apr 07, 2021
Contact name Igor I. Slukvin
Organization name University of Wisconsin
Street address 1220 Capitol Court
City Madison
ZIP/Postal code 53715
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (12)
GSM4159483 ATACseq_D4mDOXRep1
GSM4159484 ATACseq_D4mDOXRep2
GSM4159485 ATACseq_D4pDOXRep1
BioProject PRJNA589309
SRA SRP229741

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Supplementary file Size Download File type/resource 610.9 Mb (ftp)(http) BW
GSE140341_RAW.tar 849.7 Mb (http)(custom) TAR (of BED, BW, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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