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| Status |
Public on Jan 06, 2021 |
| Title |
Profiling of melocular mechanism of SOX17 effect during hematopoieisis of human ES cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Here, using SOX17-knockout and SOX17-inducible human PSCs, paired with cell biology and molecular profiling studies, we revealed that SOX17 represents a critical upstream factor that is required for activation and linkage of HOXA and arterial programs in hemogenic endothelium and establishing definitive lympho-myeloid hematopoiesis. These SOX17 effects are mediated through activation of NOTCH, CDX2 and retinoic acid signaling. Collectively, these findings are important to design strategies for direct HSC fate programming from hPSCs.
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| Overall design |
For ATAC-seq, DNA profiles of overexpression of SOX17 and non-overexpressed SOX17 were prepared in duplicate. For ChIP-seq, SOX17 and IgG antibodies in PHE. For RNA-seq, mRNA profiles of overexpression of SOX17 and non-overexpressed SOX17 were generated by sequencing, in triplicate.
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| Contributor(s) |
Jung HS, Liu P, Ong IM, Slukvin II |
| Citation(s) |
33596423 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL142665 |
Molecular Determinants of Hemogenic Endothelium |
UNIVERSITY OF WISCONSIN MADISON |
Igor I. Slukvin |
| U01 HL134655 |
Transplantation of MHC Homozygous Vascular Progenitors in Primates |
MORGRIDGE INSTITUTE FOR RESEARCH |
James Alexander Thomson |
| P30 CA014520 |
UW Comprehensive CANCER CENTER SUPPORT |
UNIVERSITY OF WISCONSIN MADISON |
HOWARD H. BAILEY |
| P51 OD011106 |
Wisconsin National Primate Research Center Support |
UNIVERSITY OF WISCONSIN MADISON |
MARSHA RUTH MAILICK |
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| Submission date |
Nov 13, 2019 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Igor I. Slukvin |
| Organization name |
University of Wisconsin
|
| Street address |
1220 Capitol Court
|
| City |
Madison |
| ZIP/Postal code |
53715 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA589309 |
| SRA |
SRP229741 |
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