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| Status |
Public on Sep 28, 2020 |
| Title |
Single-cell RNA sequencing on EM-E6/E7/TERT-1 endometrial epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the single-cell high-throughput sequencing for non-tumorigenic EM-E6/E7/TERT-1(EME6/7t) endometrial epithelial cell line. EME6/7t cells were exposed to condition medium (CM) of adipose-derived stromal cells (ASCs) with or without tiplaxtinin (PAI-039). We employed unsupervised clustering analysis to characterize various PAI-1 related regulatory models in EME6/7t cells. This study provides a framework for exploring heterogeneous gene expression patterns caused by plasminogen activator inhibitor-1 (PAI-1) responses in the tumor microenvironment.
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| Overall design |
Single-cell mRNA profiles of 1,911 EME6/7t cells were used to study and analyze PAI-1 responses in the development of endometrial cancer
Please note that [1] the raw R1 reads was used to demultiplex row barcodes of column samples, and alignment was done on R2 reads. Therefore only R2 was provided and processed as single [2] the 'raw_to_processed_data_file.txt' contains the list of the raw data files associated with each processed data file.
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| Contributor(s) |
Huang TH, Kirma NB, Lin L, Lin C |
| Citation(s) |
33053339 |
| Submission date |
Nov 08, 2019 |
| Last update date |
Nov 09, 2020 |
| Contact name |
Tim Hui-Ming Huang |
| Organization name |
University of Texas Health at San Antonio
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| Street address |
7703 Floyd Curl Dr
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| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA588396 |
| SRA |
SRP229340 |
