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Series GSE140130 Query DataSets for GSE140130
Status Public on Sep 28, 2020
Title Single-cell RNA sequencing on EM-E6/E7/TERT-1 endometrial epithelial cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report the single-cell high-throughput sequencing for non-tumorigenic EM-E6/E7/TERT-1(EME6/7t) endometrial epithelial cell line. EME6/7t cells were exposed to condition medium (CM) of adipose-derived stromal cells (ASCs) with or without tiplaxtinin (PAI-039). We employed unsupervised clustering analysis to characterize various PAI-1 related regulatory models in EME6/7t cells. This study provides a framework for exploring heterogeneous gene expression patterns caused by plasminogen activator inhibitor-1 (PAI-1) responses in the tumor microenvironment.
Overall design Single-cell mRNA profiles of 1,911 EME6/7t cells were used to study and analyze PAI-1 responses in the development of endometrial cancer

Please note that [1] the raw R1 reads was used to demultiplex row barcodes of column samples, and alignment was done on R2 reads. Therefore only R2 was provided and processed as single [2] the 'raw_to_processed_data_file.txt' contains the list of the raw data files associated with each processed data file.
Contributor(s) Huang TH, Kirma NB, Lin L, Lin C
Citation(s) 33053339
Submission date Nov 08, 2019
Last update date Nov 09, 2020
Contact name Tim Hui-Ming Huang
Organization name University of Texas Health at San Antonio
Street address 7703 Floyd Curl Dr
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (3)
GSM4154727 HT19111001_Con1
GSM4154728 HT19111002_CM1
GSM4154729 HT19111003_CMI1
BioProject PRJNA588396
SRA SRP229340

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140130_RAW.tar 3.8 Gb (http)(custom) TAR (of TXT)
GSE140130_raw_to_processed_data_file.txt.gz 106.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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