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Series GSE14002 Query DataSets for GSE14002
Status Public on Feb 10, 2009
Title J82 human bladder cell line treated with frankincense oil
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Frankincense oil is prepared from aromatic hardened wood resin obtained by tapping Boswellia trees. For thousands of years, it has been important both socially and economically as an ingredient in incense and perfumes. Frankincense oil is a botanical oil distillate made from fermented plants that contains boswellic acid, a component known to have anti-neoplastic properties. We evaluated frankincense oil-induced cytotoxicity in bladder cancer cells. With a window of concentration, frankincense oil suppressed cell viability and induced cytotoxicity in bladder transitional carcinoma J82 cells but not normal bladder urothelial UROtsa cells immortalized with SV40 large T antigen. However, frankincense oil-induced J82 cell death did not result in DNA fragmentation. Microarray and bioinformatics analysis confirmed that frankincense oil activated cell cycle arrest, suppressed cell proliferation, and activated apoptosis in J82 cells through a series of potential pathways. These finding suggest that bladder cancer can be treated through intravesical administration of pharmaceutical agents similar to direct application on melanoma.
 
Overall design 2E05 J82 cells were untreated or treated with a 1/1000 dilution of frankincense oil for 0.5, 1, 2, or 3 hours prior to RNA extraction.
 
Contributor(s) Frank MB, Yang Q, Osban J, Azzarello J, Saban M, Saban R, Welter J, Lin H
Citation(s) 19296830
Submission date Dec 16, 2008
Last update date Mar 20, 2017
Contact name Bart Frank
E-mail(s) frank@omrf.org
Organization name OMRF
Street address 825 NE 13 St
City Oklahoma City
State/province OK
ZIP/Postal code 73103
Country USA
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (5)
GSM351684 Sample 66: untreated
GSM351685 Sample 67: treated with frankincense oil for 0.5 hr
GSM351686 Sample 68: treated with frankincense oil for 1 hr
Relations
BioProject PRJNA111267

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14002_RAW.tar 4.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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