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Series GSE139997 Query DataSets for GSE139997
Status Public on Oct 04, 2022
Title Effects of Different Doses of rAAV 5-mCherry on Transcriptome of Hippocampal Pyramidal Neurons in Male Mouse
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Here, we evaluated the effects of three different doses of rAAV5 vectors, which are widely used for functional studies in the brain, on hippocampal pyramidal neurons transcription in male mice.
Results: There was a strong correlation between rAAV dose and toxicity. As dose increased, rAAV5-DIO-mCherry upregulated genes related to the immune and inflammation response and neuron death and downregulated genes associated with the synapse and axon compartments, mitochondrial potein complex suggesting that higher dose rAAV 5-DIO-mCherry may induce pyramidal neuronal fragility and impair synaptic transmission and structure.
Conclusions:the dose should be taken into consideration when rAAV vectors are used for gene transduction studies.
Overall design nine samples were analyzed. there three groups,and three biological replication for each group
Contributor(s) Liu Y, Chen Y, Li Z, chen H, Hu N, Yang J, Gao T
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 06, 2019
Last update date Oct 06, 2022
Contact name YISI LIU
Street address SHATAI ROAD
ZIP/Postal code 510000
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (9)
GSM4151314 S1001
GSM4151315 S1002
GSM4151316 S1003
BioProject PRJNA587942
SRA SRP228768

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139997_RAW.tar 2.1 Mb (http)(custom) TAR (of TXT)
GSE139997_all.fpkm.xls.gz 2.0 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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