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Series GSE139904 Query DataSets for GSE139904
Status Public on Jan 27, 2022
Title Single cell RNA sequencing of peripheral retina in control and FGF signaling mutant mouse embryonic tissue
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of the study is to identify changes in gene expression and cell distribution in the ciliary margin upon loss of FGF signaing. Neural retinal cells from embryonic day E14 were isolated from control and mutant eyes. Dissociated cells were sorted based on GFP and TdTomato reporter expression in the control and mutant retina respectively. Flow-sorted single cells were processed by the core facility at Columbia University on a 10x platform.
Overall design 3 control and 3 mutant embryos were pooled and sorted based on reporter expression for single cell sequencing
Contributor(s) Balasubramanian R, Zhang X
Citation(s) 34757798
Submission date Nov 04, 2019
Last update date Aug 07, 2023
Contact name Revathi Balasubramanian
Organization name Columbia University
Department Ophthalmology
Street address 635 West 165th street
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (1)
GSM4148508 FGF control and mutant
BioProject PRJNA587503
SRA SRP228556

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139904_RAW.tar 774.6 Mb (http)(custom) TAR (of H5, MTX, TSV)
GSE139904_analysis.tar.gz 30.2 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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