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| Status |
Public on Jun 17, 2020 |
| Title |
Single-cell transcriptomic analysis of human germinal center B cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In response to T cell-dependent antigens, mature B cells are stimulated to form germinal centers, which are the place of B cell affinity maturation and selection. Here, we dissected the heterogeneity of germinal center B cells and reconstructed their pathway of differentiation using single-cell transcriptomic analysis.
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| Overall design |
Dark zone, light zone and total germinal center B cells were isolated from two donors and analyzed by single-cell RNAseq. Total germinal center B cells were isolated from a third donor and subject to Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq).
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| |
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| Contributor(s) |
Basso K, Holmes AB, Dalla-Favera R |
| Citation(s) |
32603407 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P30 CA013696 |
DEVELOPMENTAL FUNDS |
Trustees of Columbia University in the City of New York |
RICCARDO DALLA-FAVERA |
|
| Submission date |
Nov 04, 2019 |
| Last update date |
Jul 02, 2020 |
| Contact name |
Riccardo Dalla-Favera |
| E-mail(s) |
rd10@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
1130 St Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA587486 |
| SRA |
SRP228546 |
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