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Series GSE139891 Query DataSets for GSE139891
Status Public on Jun 17, 2020
Title Single-cell transcriptomic analysis of human germinal center B cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In response to T cell-dependent antigens, mature B cells are stimulated to form germinal centers, which are the place of B cell affinity maturation and selection. Here, we dissected the heterogeneity of germinal center B cells and reconstructed their pathway of differentiation using single-cell transcriptomic analysis.
Overall design Dark zone, light zone and total germinal center B cells were isolated from two donors and analyzed by single-cell RNAseq. Total germinal center B cells were isolated from a third donor and subject to Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq).
Contributor(s) Basso K, Holmes AB, Dalla-Favera R
Citation(s) 32603407
NIH grant(s)
Grant ID Grant title Affiliation Name
P30 CA013696 DEVELOPMENTAL FUNDS Trustees of Columbia University in the City of New York RICCARDO DALLA-FAVERA
Submission date Nov 04, 2019
Last update date Jul 02, 2020
Contact name Riccardo Dalla-Favera
Organization name Columbia University
Street address 1130 St Nicholas Avenue
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (10)
GSM4148370 GC1
GSM4148371 GC2
GSM4148372 DZ1
BioProject PRJNA587486
SRA SRP228546

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Supplementary file Size Download File type/resource
GSE139891_RAW.tar 88.0 Mb (http)(custom) TAR (of CSV, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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