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Series GSE139850 Query DataSets for GSE139850
Status Public on Feb 14, 2022
Title Identification of a retinoic acid-dependent definitive hematopoietic progenitor from human pluripotent stem cells [scRNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The generation of the hematopoietic stem cells (HSCs) from human pluripotent stem cells (hPSCs) is a major goal for regenerative medicine. HSCs derive from hemogenic endothelium (HE) in a retinoic acid (RA)-dependent manner1. While a WNT-dependent (WNTd) patterning of nascent mesoderm specifies clonally multipotent definitive HE from hPSCs2,3, this HE is functionally unresponsive to RA4. Here we show that WNTd mesoderm, prior to HE specification, is comprised of two distinct KDR+CD34negT+ populations. In a TGFb-independent manner, CXCR4negCYP26A1+ mesoderm is specified, which gives rise to HOXA+ multilineage definitive HE, in an RA-independent manner. In contrast, CXCR4+ALDH1A2+ mesoderm is specified in a TGFb-dependent manner, and gives rise to multilineage definitive HE in a stage-specific, RA-dependent manner. Further, this NOTCH-dependent HE harbored HOXA expression resembling fetal HE. This model of human hematopoietic development defines the mesodermal origins of multiple waves of hematopoiesis, and that RA-dependent hematopoietic development occurs prior to HE development.
Overall design Whole day 3 embroid bodies (EBs) from WNTi (IWP2 + ACTIVIN A) and WNTd (CHIR99021 + SB43152) were harvest and processed using the 10X platform.
Contributor(s) Luff SA, Creamer JP, Valsoni S, Dege C, Scarfo R, Dacunto A, Merelli I, Morris S, Ditadi A, Sturgeon CM
Citation(s) 35569347
Submission date Nov 01, 2019
Last update date Jul 07, 2022
Contact name Stephanie Luff
Organization name Icahn School of Medicine at Mount Sinai
Department Cell, Developmental, & Regenerative Biology
Lab Sturgeon
Street address 1425 Madison Ave
City New York
State/province NY
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (2)
GSM4147343 IWP2
GSM4147344 CHIR
This SubSeries is part of SuperSeries:
GSE139853 Identification of a retinoic acid-dependent definitive hematopoietic progenitor from human pluripotent stem cells
BioProject PRJNA587149
SRA SRP227988

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Supplementary file Size Download File type/resource
GSE139850_counts_matrix.txt.gz 42.9 Mb (ftp)(http) TXT
GSE139850_metadata.txt.gz 239.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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