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Series GSE139842 Query DataSets for GSE139842
Status Public on Nov 02, 2019
Title Transcriptomics analysis of cortical astrocytes subjected to Rbpj-K deletion and stab-wound injury
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed single cell RNA-sequencing to investigate the molecular properties of Rbpj-K deleted astrocytes from the mouse somatosensory cortex. In the presence of a stab-wound injury, these cells undertake a neurogenic program, which can be analysed in the present dataset. The experiment encompassed cells from Rbpj-K-/- as well as wild type animals, and collected from injured or healthy cortices 3 wpi.
Overall design Examination of transcriptional changes occurring in cortical astrocytes isolated from the mouse cortex subjected to Rbpj-K deletion and stab-wound injury. Four samples were analyzed: WT x healthy; WT x injured; KO x healthy; KO x injured.
Contributor(s) Zamboni M, Frisén J
Citation(s) 32758425
Submission date Nov 01, 2019
Last update date Aug 13, 2020
Contact name Margherita Zamboni
Organization name Karolinska Institutet
Department Cell and Molecular Biology
Lab Jonas Frisen
Street address Solnavägen 9
City Stockholm
ZIP/Postal code 171 65
Country Sweden
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (5)
GSM4147273 healthy_wt_10X_18_015
GSM4147274 injured_wt_10X_18_016
GSM4147275 healthy_hom_10X_18_018
BioProject PRJNA587138
SRA SRP227978

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Supplementary file Size Download File type/resource
GSE139842_all_cells_10X_gbm.csv.gz 12.5 Mb (ftp)(http) CSV
GSE139842_filtered_10X_gbm.csv.gz 8.5 Mb (ftp)(http) CSV
GSE139842_meta_all_cells.csv.gz 255.3 Kb (ftp)(http) CSV
GSE139842_meta_filtered.csv.gz 150.4 Kb (ftp)(http) CSV
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