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Series GSE139838 Query DataSets for GSE139838
Status Public on Feb 14, 2022
Title Identification of a retinoic acid-dependent definitive hematopoietic progenitor from human pluripotent stem cells [CXCR4]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The generation of the hematopoietic stem cells (HSCs) from human pluripotent stem cells (hPSCs) is a major goal for regenerative medicine. HSCs derive from hemogenic endothelium (HE) in a retinoic acid (RA)-dependent manner1. While a WNT-dependent (WNTd) patterning of nascent mesoderm specifies clonally multipotent definitive HE from hPSCs2,3, this HE is functionally unresponsive to RA4. Here we show that WNTd mesoderm, prior to HE specification, is comprised of two distinct KDR+CD34negT+ populations. In a TGFb-independent manner, CXCR4negCYP26A1+ mesoderm is specified, which gives rise to HOXA+ multilineage definitive HE, in an RA-independent manner. In contrast, CXCR4+ALDH1A2+ mesoderm is specified in a TGFb-dependent manner, and gives rise to multilineage definitive HE in a stage-specific, RA-dependent manner. Further, this NOTCH-dependent HE harbored HOXA expression resembling fetal HE. This model of human hematopoietic development defines the mesodermal origins of multiple waves of hematopoiesis, and that RA-dependent hematopoietic development occurs prior to HE development.
Overall design 6 hPSC-derived mesodermal samples underwent whole-transcriptome analysis. 3 of the samples are KDR+CXCR4- and the other 3 are KDR+CXCR4+
Contributor(s) Luff SA, Creamer JP, Dege C, Scarfo R, Dacunto A, Merelli I, Morris S, Ditadi A, Sturgeon CM
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Submission date Nov 01, 2019
Last update date Feb 14, 2022
Contact name Stephanie Luff
Organization name Icahn School of Medicine at Mount Sinai
Department Cell, Developmental, & Regenerative Biology
Lab Sturgeon
Street address 1425 Madison Ave
City New York
State/province NY
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM4147210 KDR+CXCR4- replicate #1
GSM4147211 KDR+CXCR4- replicate #2
GSM4147212 KDR+CXCR4- replicate #3
This SubSeries is part of SuperSeries:
GSE139853 Identification of a retinoic acid-dependent definitive hematopoietic progenitor from human pluripotent stem cells
BioProject PRJNA587131
SRA SRP227974

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139838_FPKM.txt.gz 575.2 Kb (ftp)(http) TXT
GSE139838_counts.txt.gz 211.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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