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| Status |
Public on Apr 24, 2020 |
| Title |
Single-Cell transcriptomes and immune profiling of IgG+ murine memory B cells from Bone Marrow and Spleen. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell sequencing for analysis gene of gene transcription and Ig repertoires of isotype-switched IgG-expressing memory B cells for paired analysis with Ig repertoires in the same cells. Aim of the study was to explore heterogeneity of isotype-switched memory B cells within and between spleen and bone marrow compartments.
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| Overall design |
Sequencing of RNA and Ig was performed in duplicates of IgG+ B cells isolated from spleen and bone marrow of C57BL/6 mice.
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| Contributor(s) |
Riedel R, Addo RK, Heinz GA, Heinrich F, Durek P, Mashreghi M, Radbruch A |
| Citation(s) |
32444631 |
| |
| Submission date |
Nov 01, 2019 |
| Last update date |
May 28, 2020 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
|
| Organization name |
Deutsches Rheuma-Forschungszentrum
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE140133 |
Single-Cell transcriptomes and BCR sequences of IgG+ murine memory B cells from Bone Marrow and Spleen |
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| Relations |
| BioProject |
PRJNA587129 |
| SRA |
SRP227973 |
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