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Series GSE139639 Query DataSets for GSE139639
Status Public on Jul 08, 2021
Title Postnatal memory governs cell function in adult skeletal muscle.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Skeletal muscle is the contractile tissue that distributes throughout body, with its functionally heterogeneous properties amongst muscles, which may relate to region specific pathology of muscle diseases. Here we found that homeobox (Hox) genes, key regulators of body-plan in the embryo, are region-specifically and robustly maintained as positional memory in both muscle and its associated stem cells named satellite cells in adult mice and humans. Although satellite cell function in adults was unaffected by genetic loss of Hoxa10 in muscle progenitors at the developmental stage, postnatal deletion of Hoxa10 in satellite cells led to genomic instability and mitosis abnormality, resulting in a remarkable decline in regenerative ability of muscles in a region-specific manner. Our results suggest that Hox-based positional memory is flexibly established during embryonic development and governs topographic function of stem cells in adult muscles once it is fixed, potentially influencing the region-specific weakness in muscle diseases
Overall design Proliferating satellite cell mRNA profiles of CON (Hoxa10f/f) and scKO (Pax7-CreERT2/+;Hoxa10f/f) were generated by RNA-seq.
Contributor(s) Kiyoshi Y, Jumpei N, Yasuyuki O, Yusuke O
Citation(s) 34108202
Submission date Oct 31, 2019
Last update date Jul 08, 2021
Contact name Yasuyuki Ohkawa
Organization name Medical Institute of Bioregulation
Lab Division of Transcriptomics
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 8128582
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (18)
GSM4145032 control rep1
GSM4145033 control rep2
GSM4145034 control rep3
BioProject PRJNA586756
SRA SRP227752

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Supplementary file Size Download File type/resource
GSE139639_umi_counts.txt.gz 503.3 Kb (ftp)(http) TXT
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