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Series GSE139593 Query DataSets for GSE139593
Status Public on May 05, 2020
Title Cell Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the transcriptional responses to heat shock of inner ear sensory hair cells and supporting cells, we performed cell-type-specific transcriptional profiling using the RiboTag method, which allows for immunoprecipitation of actively translating mRNAs from specific cell types. RNA-Seq differential gene expression analyses demonstrated that RiboTag identified known cell type-specific markers as well as new markers for hair cells and supporting cells. Gene expression differences suggest that both hair cells and supporting cells exhibit a transcriptional heat shock response. However, hair cells and supporting cells expressed different members of the heat shock protein family in response to heat stress, and supporting cells expressed a larger number of HSPs. Only one HSP, Chaperonin Containing T-Complex Polypeptide 1 Subunit 8, (CCT8) was uniquely induced in hair cells. Together our data indicate that hair cells exhibit a limited but unique heat shock response, and supporting cells exhibit a broader and more robust transcriptional response to protective heat stress.
Overall design RNA-seq comparisons of control (CON) versus Heat-shock treated cultured utricles. The RNA was isolated fom ribotag-enriched immunoprecipates (IP) and compared to RNA isolated from input to identify genes enriched in the IP. Two cre-expressing mouse lines were used to obtain either hair-cell enriched IP samples (Gfi1-Cre) or supporting cell enrich IP samples (GLAST-CreER).
Contributor(s) Sadler E, Ryals MR, May LA, Martin D, Welsh N, Boger ET, Morell RJ, Hertzano RP, Cunningham LL
Citation(s) 32528249
Submission date Oct 30, 2019
Last update date Nov 21, 2022
Contact name Robert Morell
Organization name National Institute on Deafness and Other Communication Disorders
Street address 35A Convent Drive, Room 1F-103
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (34)
GSM4144257 Gfi1_Con_input_1
GSM4144258 Gfi1_Con_input_3
GSM4144259 Gfi1_Con_input_4
BioProject PRJNA580444
SRA SRP227469

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139593_GLAST-creER_enriched_genes.txt.gz 22.0 Kb (ftp)(http) TXT
GSE139593_Gfi1-cre_enriched_genes.txt.gz 19.9 Kb (ftp)(http) TXT
GSE139593_RAW.tar 10.1 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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