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Series GSE139527 Query DataSets for GSE139527
Status Public on Nov 01, 2020
Title Reprogramming of the histone H3.3 landscape in the early mouse embryo
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Epigenetic reprogramming of the zygote involves dynamic incorporation of the histone variant, H3.3. However, the genome-wide distribution and dynamics of H3.3 during early development remain unknown. Here, we delineate the H3.3 landscapes in mouse oocytes and early embryos. We unexpectedly identify a non-canonical H3.3 pattern in mature oocytes and zygotes, in which local enrichment of H3.3 at active chromatin is suppressed and H3.3 is relatively evenly distributed across the genome. Interestingly, while the non-canonical H3.3 pattern forms gradually during oogenesis, it quickly switches to a canonical pattern at the 2-cell stage in a transcription-independent and replication-dependent manner. We find that incorporation of H3.1/H3.2 mediated by CAF-1 is a key process for the de novo establishment of the canonical pattern. Our data suggest that the presence of the non-canonical pattern and its timely transition toward a canonical pattern support the developmental program of early embryos.
Overall design H3.3 ChIP-seq was performed with mouse oocytes, early embryos and mESCs. H3.1/H3.2 CUT&RUN was performed with 2-cell embryos, and H3.3 CUT&RUN was performed with mESCs. RNA-seq was performed with 8-cell embryos or blastocysts.
Contributor(s) Ishiuchi T
Citation(s) 33169018
Submission date Oct 29, 2019
Last update date Feb 08, 2021
Contact name Takashi Ishiuchi
Organization name Kyushu University
Department Medical Institute of Bioregulation
Lab Epigenetics and Development
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (4)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (73)
GSM4142946 MII oocyte input rep1
GSM4142947 MII oocyte H3.3 ChIP-seq rep1
GSM4142948 Zygote input rep1
BioProject PRJNA580180
SRA SRP227310

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Supplementary file Size Download File type/resource 103.6 Mb (ftp)(http) BW 156.9 Mb (ftp)(http) BW 148.9 Mb (ftp)(http) BW 159.9 Mb (ftp)(http) BW
GSE139527_All_samples_FPKM.txt.gz 990.6 Kb (ftp)(http) TXT 22.8 Mb (ftp)(http) BW 122.8 Mb (ftp)(http) BW 154.4 Mb (ftp)(http) BW 167.3 Mb (ftp)(http) BW 158.9 Mb (ftp)(http) BW 173.6 Mb (ftp)(http) BW 159.0 Mb (ftp)(http) BW 14.9 Mb (ftp)(http) BW 153.5 Mb (ftp)(http) BW 167.4 Mb (ftp)(http) BW 163.3 Mb (ftp)(http) BW 15.9 Mb (ftp)(http) BW 16.2 Mb (ftp)(http) BW 22.4 Mb (ftp)(http) BW 15.6 Mb (ftp)(http) BW 15.9 Mb (ftp)(http) BW
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