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Series GSE139495 Query DataSets for GSE139495
Status Public on Aug 25, 2020
Title Analysis of Circulating Breast Cancer Cell Heterogeneity and Interactions with Peripheral Blood Mononuclear Cells.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To infer interactions between circulating breast cancer cells and peripheral mononuclear cells using bioinformatics analysis of single cell RNA-sequencing.
Methods: Filter based method for capturing single cells from 7.5mL of blood from patients with breast cancer, followed by single cell RNA-sequencing.
Results: Transcriptomes predicted for two CTC populations with distinct expression profiles and interactions with peripheral blood mononuclear cells.
Overall design 10 samples from breast cancer patients (9 with metastatic breast cancer and 1 with stage 2 local breast cancer). A total of 7.5 mL of blood from each sample was used to capture single cells for single cell RNA-sequencing.
Contributor(s) Kabos P, Brechbuhl HM, Paul KV, Gillen AE
Citation(s) 32822091
Submission date Oct 28, 2019
Last update date Aug 25, 2020
Contact name Kiran Vinod Paul
Organization name University of Colorado
Street address 12801 E 17th Ave RC1 South 8401M
City Aurora
State/province Colorado
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (9)
GSM4142367 2 Metastatic ER+ breast cancer
GSM4142368 3 Metastatic ER+ breast cancer
GSM4142369 5 Metastatic ER+ breast cancer
BioProject PRJNA579983
SRA SRP227241

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Supplementary file Size Download File type/resource
GSE139495_Single_cell_Raw_counts_10252019.tsv.gz 17.5 Mb (ftp)(http) TSV
GSE139495_Single_cell_metaData_10252019.tsv.gz 224.7 Kb (ftp)(http) TSV
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