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| Status |
Public on Aug 25, 2020 |
| Title |
Analysis of Circulating Breast Cancer Cell Heterogeneity and Interactions with Peripheral Blood Mononuclear Cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To infer interactions between circulating breast cancer cells and peripheral mononuclear cells using bioinformatics analysis of single cell RNA-sequencing.
Methods: Filter based method for capturing single cells from 7.5mL of blood from patients with breast cancer, followed by single cell RNA-sequencing.
Results: Transcriptomes predicted for two CTC populations with distinct expression profiles and interactions with peripheral blood mononuclear cells.
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| Overall design |
10 samples from breast cancer patients (9 with metastatic breast cancer and 1 with stage 2 local breast cancer). A total of 7.5 mL of blood from each sample was used to capture single cells for single cell RNA-sequencing.
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| Contributor(s) |
Kabos P, Brechbuhl HM, Paul KV, Gillen AE |
| Citation(s) |
32822091 |
| Submission date |
Oct 28, 2019 |
| Last update date |
Aug 25, 2020 |
| Contact name |
Kiran Vinod Paul |
| E-mail(s) |
kiran.vinodpaul@cuanschutz.edu
|
| Organization name |
University of Colorado
|
| Street address |
12801 E 17th Ave RC1 South 8401M
|
| City |
Aurora |
| State/province |
Colorado |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA579983 |
| SRA |
SRP227241 |
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