GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE139462 Query DataSets for GSE139462
Status Public on May 11, 2020
Title Selective Mediator-dependence of cell type-specifying transcription [PRO-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The Mediator complex directs signals from DNA-binding transcription factors to RNA polymerase (Pol) II. Despite this pivotal position, mechanistic understanding of Mediator in human cells remains incomplete. Here, we quantified Mediator-controlled Pol II kinetics by coupling rapid subunit degradation with orthogonal experimental readouts. Consistent with a model of condensate-driven transcription initiation, large clusters of hypo-phosphorylated Pol II rapidly disassembled upon Mediator degradation. This was accompanied by a selective and pronounced disruption of cell type-specifying transcriptional circuits, whose constituent genes featured exceptionally high rates of Pol II turnover. Notably, transcriptional output of most other genes was largely unaffected by acute Mediator ablation. Maintenance of transcriptional activity at these genes was linked to an unexpected, CDK9-dependent compensatory feedback loop that elevated Pol II pause release rates genome-wide. Collectively, our work positions human Mediator as a globally acting coactivator that selectively safeguards the functionality of cell type-specifying transcriptional networks.
Overall design PRO-seq in KBM7 MED14-dTAG cells after 1-2h 500nM dTAG7 and/or 30min 500nM NVP2.
Contributor(s) Jaeger MG, Winter GE
Citation(s) 32483291
Submission date Oct 28, 2019
Last update date Aug 17, 2020
Contact name Georg E Winter
Organization name CeMM - Research Institute for Molecular Medicine of the Austrian Academy of Sciences
Street address Lazarettgasse 14, AKH BT25.3
City Wien
State/province Austria
ZIP/Postal code 1090
Country Austria
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (16)
GSM4141983 PRO-seq_MJ-19-14_MED14-dTAG_1h_DMSO_A
GSM4141984 PRO-seq_MJ-19-14_MED14-dTAG_1h_DMSO_B
GSM4141985 PRO-seq_MJ-19-14_MED14-dTAG_1h_dTAG7_A
This SubSeries is part of SuperSeries:
GSE139468 Selective Mediator dependence of cell-type-specifying transcription
BioProject PRJNA579916
SRA SRP227189

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139462_RAW.tar 2.7 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap