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Series GSE139342 Query DataSets for GSE139342
Status Public on Jun 19, 2020
Title Single cell RNA-seq revealed different cell types induced by loss of ARID1A in undifferentiated and differentiation (day 10) H9 hESCs.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We reported loss of ARID1A promoted neurogenesis and inhibited cardiogenesis. Under microscopy, we observed that spontaneously differentiated cells were induced in ARID1A KO H9 hESCs cultured in mTesR medium. After cardiac differentiation for 10 days, we also observed the cell types were totally different between WT and ARID1A KO cells. We did not know what cells types were. Here scRNA-seq were used to identify the cell types in WT H9 hESCs and ARID1A KO H9 hESCs.
Overall design Identification of different cell types induced by loss of ARID1A in undifferentiated and differentiated H9 hESCs.
Contributor(s) Yang L, Liu J
Citation(s) 32646524
Submission date Oct 24, 2019
Last update date Jul 16, 2020
Contact name Lei Yang
Phone (317) 278-5233
Organization name Indiana University School of Medicine
Department Department of Pediatrics
Street address 1044 W. Walnut St.
City Indianapolis
State/province Indiana
ZIP/Postal code 46202
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM4138489 WT_undiff_scRNA-seq
GSM4138490 ARID1A KO_undiff_scRNA-seq
GSM4138491 WT_diff T10_scRNA-seq
This SubSeries is part of SuperSeries:
GSE139343 Essential and Opposite Roles of ARID1A in Coordinating Human Cardiogenesis and Neurogenesis from Pluripotent Stem Cells
BioProject PRJNA579351
SRA SRP226855

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139342_RAW.tar 172.5 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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