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Series GSE139225 Query DataSets for GSE139225
Status Public on Jun 30, 2020
Title RNAseq on Dengue specific mice immunoglobulin repertoires
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This study aims to understand the characteristics and diversification of Dengue specific immunoglobulin repertoires after different immunization strategies in mouse. We propose two different immunization strategies in dengue vaccine development. One is tetravalent strategy, which pre-mixed four serotype dengue DNA vaccine and administrated four times. The other one is sequential strategy, which sequentially administrated four time, each serotype vaccine for each dose as sequences of Den1, Den2, Den3 and Den4.
Overall design C57BL/6J (B6) mice were injected Dengue Envelope DNA vaccine with either tetravalent or sequential strategy. The Dengue specific B cells were fished out from immunized mice splenocytes by Dengue Env protein probes. After cDNA and Ig specific amplification, the RNA-seq libraries were prepared and subjected to MiSeq V3 2 × 301 bp sequencing.
Contributor(s) Chen J, Hou J
Citation(s) 32728482
Submission date Oct 21, 2019
Last update date Aug 03, 2020
Contact name Guangan Hu
Organization name MIT
Department Koch Institute
Lab Chen
Street address 500 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (8)
GSM4134338 Tetra_1st
GSM4134339 Seq_1st
GSM4134340 Tetra_2nd
BioProject PRJNA578805
SRA SRP226549

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Supplementary file Size Download File type/resource
GSE139225_Processed_Data_AllDb.csv.gz 102.5 Mb (ftp)(http) CSV
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