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Series GSE139200 Query DataSets for GSE139200
Status Public on Oct 24, 2019
Title Single cell RNAseq of adoptively transferred Tregs in rhesus macaques
Organism Macaca mulatta
Experiment type Expression profiling by high throughput sequencing
Summary Animal R.401 was infused with ex-vivo expanded T-regulatory cells. After adoptive transfer, PBMC were obtained and stained with surface antibodies. Single cell libraries from sorted populations were generated using a UMI-based droplet-partitioning platform (10X Genomics) and sequenced using a NextSeq 500 (Illumina). Resulting reads were processed using Cellranger software (10X Genomics) and downstream analysis was performed using Monocle6,7 using a negative binomial model of distribution with fixed variance.
Overall design Time-course
Contributor(s) Furlan S
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BioProject PRJNA577285
Submission date Oct 21, 2019
Last update date Oct 24, 2019
Contact name Geoff R Hill
Organization name Fred Hutchinson Cancer Research Center
Department CRD
Lab Hill Lab
Street address 1100 Fairview Ave. N.
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platforms (1)
GPL21120 Illumina NextSeq 500 (Macaca mulatta)
Samples (8)
GSM4133869 CFSEpos_D3
GSM4133870 Endo_D3
GSM4133871 CD127posCD25neg_D3
SRA SRP225421

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139200_exprs_data.txt.gz 23.8 Mb (ftp)(http) TXT
GSE139200_gene_data.txt.gz 221.2 Kb (ftp)(http) TXT
GSE139200_meta_data.txt.gz 1.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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