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Series GSE139168 Query DataSets for GSE139168
Status Public on Oct 16, 2020
Title A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study was to analyze the cellular compsitions of the arterial pole and venous pole regions of the neonatal mouse heart using single-cell RNA sequencing.
Overall design The dorsal and ventral domains of the cardiac base were dissected from 3-day old C57Bl6 male and female neonatal mice (n=15) and enzymatically dissociated into single-cell suspensions. The ventral domain included the proximal aorta, pulmonary artery, and outflow tract base. The dorsal domain included the sinoatrial node, cardiac ganglia and pulmonary veins. Single-cell preparations from 15 neonatal hearts were combined to generate two pools of dorsal and ventral cells, and cultured in vitro for 5 days to allow recovery from enzymatic and mechanical stress. Subsequently, cells were processed for 3' single cell RNA-seq using the 10X Genomics Chromium platform and sequenced on Illumina Novaseq 6000.
Contributor(s) Durante MA, Chatzistergos K
Citation(s) 33268364
Submission date Oct 21, 2019
Last update date Dec 09, 2020
Contact name Michael Durante
Phone 2393575338
Organization name University of Miami Miller School of Medicine
Department Ophthalmology
Lab Harbour
Street address 1501 NW 10th Ave., Ste 832
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM4132435 Dorsal_day3
GSM4132436 Ventral_day3
BioProject PRJNA578689
SRA SRP226460

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Supplementary file Size Download File type/resource
GSE139168_RAW.tar 384.9 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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