GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE139077 Query DataSets for GSE139077
Status Public on Oct 17, 2020
Title Two faces of bivalent domain regulate VEGFA responsiveness and angiogenesis
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The bivalent domain at promoter region is a unique epigenetic feature poised for activation or repression during cell differentiation in embryonic stem cell. However, the function of bivalent domains in already differentiated cells remains exclusive. By profiling the epigenetic landscape of endothelial cells during VEGFA stimulation, we discovered that bivalent domains are widespread in endothelial cells and preferentially marked genes responsive to VEGFA. The bivalent domains responsive to VEGFA have more permissive chromatin environment comparing to other bivalent domains. The initial activation of bivalent genes depends on RNAPII pausing release induced by EZH1 rather than removal of H3K27me3. The later suppression of bivalent gene expression depended on KDM5A recruitment by its interaction with PRC2. Importantly, EZH1 promoted both in vitro and in vivo angiogenesis by upregulating EGR3, whereas KDM5A dampened angiogenesis. Collectively, this study demonstrated a novel dual function of bivalent domains in endothelial cells to control VEGF responsiveness and angiogenesis.
Overall design Our model system consisted of human umbilical vein endothelial cells that were cultured overnight in low serum and no VEGFA, and then stimulated for 12 hours with 50 ng/ml VEGFA. Samples were collected at 0 (unstimulated), 1, 4, and 12 hours. ChIP-seq of corresponding factors or histone markers were sequenced.
Contributor(s) Chen J, Liang X, Zhang S, Wang S, Garcia SP, Yan P, Yu H, Li Z, Liu L, Wei W, Le H, Zhang Y, Yuan G, Chen S, Chen Y, Sun K, Pu WT, Zhang B
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 18, 2019
Last update date Oct 24, 2020
Contact name bing zhang
Organization name Shanghai Jiao Tong University
Department Shanghai Center for Systems Biomedicine
Lab Bing Zhang Lab
Street address 800 Dong Chuan Road
City Minhang
State/province Shanghai
ZIP/Postal code 200240
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (9)
GSM4129399 EZH1-0hour-R1
GSM4129400 EZH1-1hour-R1
GSM4129401 EZH1-4hour-R1
BioProject PRJNA578281
SRA SRP226174

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139077_RAW.tar 2.9 Gb (http)(custom) TAR (of TDF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap