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Series GSE139073 Query DataSets for GSE139073
Status Public on Nov 01, 2019
Title Multi-Parameter Analysis of Biobanked Human Bone Marrow Stromal Cells Shows Little Influence for Donor Age and Mild Comorbidities on Phenotypic and Functional Properties
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Heterogeneous populations of human bone marrow-derived stromal cells (BMSC) are among the most frequently tested cellular therapeutics for treating degenerative and immune disorders, which occur predominantly in the aging population. Currently, it is unclear whether advanced donor age and commonly associated comorbidities affect the properties of ex vivo-expanded BMSCs. Thus, we stratified cells from adult and elderly donors from our biobank (n = 10 and n = 13, mean age 38 and 72 years, respectively) and compared their phenotypic and functional performance, using multiple assays typically employed as minimal criteria for defining multipotent mesenchymal stromal cells (MSCs).We found that BMSCs from both cohorts meet the standard criteria for MSC, exhibiting similar morphology, growth kinetics, gene expression profiles, and pro-angiogenic and immunosuppressive potential and the capacity to differentiate toward adipogenic, chondrogenic, and osteogenic lineages.We found no substantial differences between cells from the adult and elderly cohorts. As positive controls, we studied the impact of in vitro aging and inflammatory cytokine stimulation. Both conditions clearly affected the cellular properties, independent of donor age. We conclude that in vitro aging rather than in vivo donor aging influences BMSC characteristics.
 
Overall design RNA-Seq transcriptome analysis of human bone marrow stromal cells at different passages from healthy and diabetic donors, with and without stimulation
 
Contributor(s) Moll G, Geissler S, Jürchott K
Citation(s) 31781089
Submission date Oct 18, 2019
Last update date Dec 03, 2019
Contact name Karsten Jürchott
E-mail(s) karsten.juerchott@charite.de
Organization name Charité - Universitätsmedizin Berlin
Department Berlin-Brandenburg Center for Regenerative Therapies
Street address Augustenburger Platz 1
City Berlin
ZIP/Postal code D-13353
Country Germany
 
Platforms (2)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (40)
GSM4128688 Bone marrow stromal cells, S1208
GSM4128689 Bone marrow stromal cells, S1207
GSM4128690 Bone marrow stromal cells, S1209
Relations
BioProject PRJNA578272
SRA SRP226171

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Supplementary file Size Download File type/resource
GSE139073_norm_data.txt.gz 4.9 Mb (ftp)(http) TXT
GSE139073_raw_counts.txt.gz 2.1 Mb (ftp)(http) TXT
GSE139073_vst_data.txt.gz 5.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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