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Series GSE139072 Query DataSets for GSE139072
Status Public on May 08, 2020
Title Nuclear Poly(A)-binding Protein 1-like (PABPN1L) Mediates Cytoplasmic mRNA Decay As a Placeholder during Maternal-to-zygotic Transition
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Maternal mRNA degradation is a critical event of maternal-to-zygotic transition (MZT) that determines the developmental potential of early embryos. Nuclear Poly(A)-binding proteins (PABPNs) are extensively involved in mRNA post-transcriptional regulations, but their functions in mammalian MZT has not been investigated. In this study, we identified a novel maternal-effect factor PABPN1-like (PABPN1L), rather than its ubiquitously expressed homolog PABPN1, as an RNA-binding adapter of the mammalian MZT licensing factor BTG4 which mediates maternal mRNAs clearance. Female Pabpn1l null mice produced morphologically normal oocytes but were infertile owing to early developmental arrest of the resultant embryos at the 1-to-2-cell stages without undergoing ZGA. Deletion of Pabpn1l impairs the deadenylation and degradation of a subset of BTG4-targeted maternal mRNAs during MZT. In addition to recruiting BTG4 to the mRNA 3ʹ-poly(A) tails, PABPN1L is also required for BTG4 protein accumulation in maturing oocytes by protecting BTG4 from SCF-βTrCP1 E3 ubiquitin ligase-mediated polyubiquitination and degradation. This study highlights a noncanonical cytoplasmic function of nuclear poly(A)-binding proteins mRNA turnover as well as its physiological importance during MZT.
Overall design GV oocytes, zygotes and 2-cell embryos of WT and Pabpn1l knockout for duplicates are performed RNA sequencing.
Contributor(s) Shen L, Zhu Y
Citation(s) 32558204
Submission date Oct 18, 2019
Last update date Aug 11, 2020
Contact name Li Shen
Phone 86-0571-88981751
Organization name Life Sciences Institute, Zhejiang University
Lab Shenlab
Street address 866 Yuhangtang Road
City Hangzhou
State/province Zhejiang Province
ZIP/Postal code 310058
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (12)
GSM4128676 Pabpn1l-WT-GV-Rep1
GSM4128677 Pabpn1l-WT-GV-Rep2
GSM4128678 Pabpn1l-WT-1Cell-Rep1
BioProject PRJNA578271
SRA SRP226170

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Supplementary file Size Download File type/resource
GSE139072_Gene_FPKM.txt.gz 615.2 Kb (ftp)(http) TXT
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