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Series GSE139017 Query DataSets for GSE139017
Status Public on Oct 01, 2022
Title Mechanisms and treatment of cold-storage-induced microvascular obstructions in normothermic machine perfusion of human kidney
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human kidneys which were accepted for transplantation and subsequently not used were subjected to ex vivo normothermic machine perfusion. During perfusion it was found the tubular epithelia synthesized fibrinogen which led to red blood cell aggregation and pathologic plugging of renal microvasculature. Sequential ex vivo delivery of plasminogen and tissue plasminogen activator (tPA) during normothermic machine perfusion effectively lyses these fibrinogen-mediated plugs.
Overall design Pairs of human kidneys were subjected to ex vivo normothermic perfusion. One kidney in each pair was treated and the other not. Samples were taken pre- and post-perfusion for RNASeq.
Contributor(s) Ferdinand JR, DiRito JR, Clatworthy MR, Nicholson ML, Tietjan GT
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Submission date Oct 17, 2019
Last update date Oct 01, 2022
Contact name John Robert Ferdinand
Organization name University of Cambridge
Street address Department of Medicine
City Cambridge
ZIP/Postal code CB2 0QH
Country United Kingdom
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM4126835 Control_1_post
GSM4126836 Control_1_pre
GSM4126837 Treatment_1_post
BioProject PRJNA578096
SRA SRP226045

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Supplementary file Size Download File type/resource
GSE139017_counts_table.csv.gz 491.6 Kb (ftp)(http) CSV
GSE139017_normcounts.csv.gz 1.6 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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