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| Status |
Public on Oct 01, 2022 |
| Title |
Mechanisms and treatment of cold-storage-induced microvascular obstructions in normothermic machine perfusion of human kidney |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human kidneys which were accepted for transplantation and subsequently not used were subjected to ex vivo normothermic machine perfusion. During perfusion it was found the tubular epithelia synthesized fibrinogen which led to red blood cell aggregation and pathologic plugging of renal microvasculature. Sequential ex vivo delivery of plasminogen and tissue plasminogen activator (tPA) during normothermic machine perfusion effectively lyses these fibrinogen-mediated plugs.
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| Overall design |
Pairs of human kidneys were subjected to ex vivo normothermic perfusion. One kidney in each pair was treated and the other not. Samples were taken pre- and post-perfusion for RNASeq.
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| Contributor(s) |
Ferdinand JR, DiRito JR, Clatworthy MR, Nicholson ML, Tietjan GT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 17, 2019 |
| Last update date |
Oct 01, 2022 |
| Contact name |
John Robert Ferdinand |
| Organization name |
University of Cambridge
|
| Street address |
Department of Medicine
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA578096 |
| SRA |
SRP226045 |
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