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Series GSE139012 Query DataSets for GSE139012
Status Public on Jul 13, 2022
Title 10x single cell sequencing for primary neurons transcriptomes after sevoflurane exposure
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We thus isolated cortex from mice, with or without sevoflurane exposure, and performed single cell sequencing. Single cell sequencing is a innovative experimental procedures which allows the identification of various subsets of cells in the neverous system after sevoflurane exposure. Isolated cells were concentrated to 1000 cells/uL and approximately 17,000 cells were loaded into each channel to generate single-cell Gel Bead-In-Emulsions (GEMs), which led to expected mRNA barcoding of 10,000 single-cells for each sample. Seurat package was used for cell normalization and regression. Sevoflurane and control groups were aligned and combined using Seurat. Using t-distributed stochastic neighbor embedding (tSNE), 13 cluters of cells were visualized. Cells were assigned to various clusters based on strong expression of specific genes.
Overall design Examination of 2 different exposure in primary neurons
Contributor(s) Zhang L
Citation(s) 33928466
Submission date Oct 17, 2019
Last update date Jul 13, 2022
Contact name yanyong cheng
Organization name Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
Department Department of Anesthesiology
Street address 639 Zhizaoju Road
City shanghai
ZIP/Postal code 200011
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4126209 CON1
GSM4126210 CON2
GSM4126211 CON3
BioProject PRJNA578089
SRA SRP226037

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Supplementary file Size Download File type/resource
GSE139012_Allsample.NormalizedCounts.txt.gz 650.5 Mb (ftp)(http) TXT
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