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| Status |
Public on Oct 31, 2019 |
| Title |
Molecular profiling of stem cell-like female germ line cells in Drosophila delineates networks important for stemness and differentiation |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We characterize the transcriptome of germline stem cell (GSC)-like cells isolated from bag of marbles (bam) mutant Drosophila ovaries by next generation RNA sequencing (RNA-seq) and compare it to the transcriptome of germline cells isolated from wild type ovaries. We further refine this dataset by utilizing an RNA-immunoprecipitation strategy to identify transcripts bound to the master differentiation factor Bam.
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| Overall design |
Transcriptomes of bam mutant and wild type (Vasa-GFP) ovaries were characterized and compared by paired-end mRNA sequencing, in triplicate. Further, transcripts bound to Bam-GFP were delineated by RNA-immunoprecipitation followed by mRNA sequencing, also in triplicate.
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| Contributor(s) |
Wodarz A |
| Citation(s) |
31649115 |
| |
| Submission date |
Oct 16, 2019 |
| Last update date |
Oct 31, 2019 |
| Contact name |
Andreas Wodarz |
| E-mail(s) |
andreas.wodarz@uk-koeln.de
|
| Organization name |
Uniklinik Cologne
|
| Department |
Anatomy
|
| Lab |
Molecular Cell Biology
|
| Street address |
Kerpener Str. 62, Building 37
|
| City |
Cologne |
| ZIP/Postal code |
50937 |
| Country |
Germany |
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| Platforms (2) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA577938 |
| SRA |
SRP225950 |
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