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Series GSE138838 Query DataSets for GSE138838
Status Public on Jan 26, 2020
Title Single cell analysis reveals human cytomegalovirus drives latently infected cells towards an anergic-like monocyte state
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human cytomegalovirus (HCMV) causes a lifelong infection through establishment of latency. Although reactivation from latency can cause life-threatening disease, our molecular understanding of HCMV latency is incomplete. Here we use single cell RNA-seq analysis to characterize latency in monocytes and hematopoietic stem and progenitor cells (HSPCs). In monocytes, we identify host cell surface markers that enable enrichment of latent cells harboring higher viral transcript levels, which can reactivate more efficiently, and are characterized by an intrinsic reduced immune response that is critical for viral gene expression. Significantly, in latent HSPCs, viral transcripts could be detected only in monocyte progenitors and were also associated with reduced immune-response. Overall, our work demonstrates that regardless of the developmental stage in which HCMV infects, HCMV drives hematopoietic cells towards a weaker immune-responsive monocyte state and that this anergic-like state is crucial for the virus ability to express its transcripts and to eventually reactivate.
Overall design MARS-Seq bulk protocol was used for monocytes-derived macrophages, monocytes FACS-sorted according to the levels of DC74, and monocytes with and without treatment by ruxolitinib, all infected with HCMV.
Contributor(s) Shnayder M, Nachshon A, Bernstein B, Fein N, Rozman B, Poole E, Lavi M, Avdic S, Blyth E, Gottlieb D, Abendroth A, Slobedman B, Sinclair J, Stern-Ginossar N, Schwartz M
Citation(s) 31967545
Submission date Oct 15, 2019
Last update date Jan 27, 2020
Contact name Noam Stern-Ginossar
Organization name Weizmann Institute
Department Molecular Genetics
Lab Noam Stern-Ginossar
Street address 234 herzel
City Rehovot
ZIP/Postal code 7610001
Country Israel
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (21)
GSM4120185 Derived macrophages 4dpi
GSM4120186 Mococytes 3dpi CD74 high A
GSM4120187 Mococytes 3dpi CD74 high B
BioProject PRJNA577574
SRA SRP225665

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Supplementary file Size Download File type/resource
GSE138838_cd74_high_vs_low.txt.gz 297.5 Kb (ftp)(http) TXT
GSE138838_macrophages.txt.gz 98.7 Kb (ftp)(http) TXT
GSE138838_ruxo_kinetics.txt.gz 390.2 Kb (ftp)(http) TXT
GSE138838_ruxo_plus_vs_minus.txt.gz 400.4 Kb (ftp)(http) TXT
GSE138838_sample_barcodes.txt.gz 340 b (ftp)(http) TXT
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