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Series GSE138824 Query DataSets for GSE138824
Status Public on Apr 18, 2020
Title Vascular smooth muscle selective deletion of Srf
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Previous efforts to conditionally delete the Srf transcription factor in smooth muscle lineages were met with early demise of the mouse due to a gastrointestinal (GI) obstruction phenotype. We have developed a new, more VSMC selective Cre recombinase mouse (Itga8-CreERT2), that circumvents the GI phenotype thus affording the opportunity to evaluate, in an unambiguous manner, VSMC phenotypes where Srf is reduced. The mice live for at least 6 months post-tamoxifen and here we report the first RNA-seq experiments in mouse aortic SMC where Srf is reduced.
Overall design Mice homozygous or heterozygous for a floxed Srf allele and hemizygous for the Itga8-CreERT2 driver were treated with 5 daily doses of 33mg/kg Tamoxifen (i.p.). Aortae from the experimental homozygous cKO (n=3) or the control heterozygous cKO (n=3) were harvested 10 days following the last tamoxifen injection. Only 2 homozygous cKO replicates are reported here due to an outlier effect (or error in sampling) in one of the experimental samples. Thus, the data herein reflect only 2 homoygous cKOs and 3 heterozygous cKO controls.
Contributor(s) Lyu Q, Zhang W, Slivano OJ, Christie CK, Long X, Gan L, Miano JM
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Submission date Oct 14, 2019
Last update date Apr 18, 2020
Contact name Joseph M Miano
Organization name University of Rochester
Street address 601 Elmwood Avenue
City Rochester
State/province NY
ZIP/Postal code 14642
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4120092 Ctrl1
GSM4120093 Ctrl2
GSM4120094 Ctrl3
BioProject PRJNA577451
SRA SRP225543

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Supplementary file Size Download File type/resource
GSE138824_deSeq2_counts.txt.gz 438.1 Kb (ftp)(http) TXT
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Processed data are available on Series record

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