 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 11, 2019 |
| Title |
Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The RNA modification N6-methyladenosine (m6A) can modulate mRNA fate and thus affect many biological processes. We analyzed m6A modification across the transcriptome following infection by dengue virus (DENV), Zika virus (ZIKV), West Nile virus (WNV), and hepatitis C virus (HCV). We found that infection by these viruses in the Flaviviridae family alters m6A modification of specific cellular transcripts, including RIOK3 and CIRBP. During viral infection, the addition of m6A to RIOK3 promotes its translation, while loss of m6A in CIRBP promotes alternative splicing. Importantly, we found that viral activation of innate immune sensing or the endoplasmic reticulum (ER) stress response contributes to the changes in m6A modification in RIOK3 and CIRBP, respectively. Further, several transcripts with infection-altered m6A profiles, including RIOK3 and CIRBP, encode proteins that influence DENV, ZIKV, and HCV infection. Overall, this work reveals that cellular signaling pathways activated during viral infection lead to alterations in m6A modification of host mRNAs to regulate infection.
|
| |
|
| Overall design |
Study of m6A changes in cellular RNA from Huh7 cells following 4 biological replicates of innate immune activation (HCV PAMP transfection, 8 hpt) or ER stress (thapsigargin (TG) treatment, 16 hpt).
|
| Web link |
https://doi.org/10.1016/j.molcel.2019.11.007
|
| |
|
| Contributor(s) |
Gokhale NS, McIntyre AB, Mattocks MD, Holley CL, Lazear HM, Mason CE, Horner SM |
| Citation(s) |
31810760 |
| Submission date |
Oct 10, 2019 |
| Last update date |
Jan 10, 2020 |
| Contact name |
A McIntyre |
| Organization name |
University of Zurich
|
| Department |
Molecular Life Sciences
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA576915 |
| SRA |
SRP225110 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE138730_PAMP_1.macs2_peaks.narrowPeak.gz |
217.3 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_PAMP_2.macs2_peaks.narrowPeak.gz |
199.9 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_PAMP_3.macs2_peaks.narrowPeak.gz |
204.3 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_PAMP_4.macs2_peaks.narrowPeak.gz |
226.9 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Pmock_1.macs2_peaks.narrowPeak.gz |
221.6 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Pmock_2.macs2_peaks.narrowPeak.gz |
222.8 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Pmock_3.macs2_peaks.narrowPeak.gz |
237.7 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Pmock_4.macs2_peaks.narrowPeak.gz |
274.0 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_TG_1.macs2_peaks.narrowPeak.gz |
118.7 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_TG_2.macs2_peaks.narrowPeak.gz |
176.0 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_TG_3.macs2_peaks.narrowPeak.gz |
216.1 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_TG_4.macs2_peaks.narrowPeak.gz |
207.4 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Tmock_1.macs2_peaks.narrowPeak.gz |
156.4 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Tmock_2.macs2_peaks.narrowPeak.gz |
168.3 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Tmock_3.macs2_peaks.narrowPeak.gz |
228.1 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE138730_Tmock_4.macs2_peaks.narrowPeak.gz |
223.4 Kb |
(ftp)(http) |
NARROWPEAK |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...