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| Status |
Public on Dec 30, 2019 |
| Title |
Mitochondrial Pyruvate Carrier 1 (MPC1) promotes peripheral T cell quiescence through metabolic regulation of thymic development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The transporter which uptakes pyruvate into the mitochondria during pyruvate oxidation, called the Mitochondrial Pyruvate Carrier (MPC), was only recently identified. While the MPC has been studied in a variety of cell types, including cancer cells and stem cells, very little is known about the importance of MPC in immune cells. Here, we delete MPC from hematopoietic cells and measure its impact on thymocyte gene expression by 10X Genomics scRNA-seq.
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| Overall design |
DAPI negative cells were FACS sorted from homogenized and RBC lysed thymi of 6-10 week old Female MPC1fl/fl (WT) and Vav-Cre MPC1fl/fl (KO). Single cell data.
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| Contributor(s) |
Ramstead AG, Wallace JA, O'Connell RM |
| Citation(s) |
32130894 |
| Submission date |
Oct 10, 2019 |
| Last update date |
Dec 05, 2022 |
| Contact name |
Ryan O'Connell |
| E-mail(s) |
ryan.oconnell@path.utah.edu
|
| Organization name |
University of Utah
|
| Department |
Pathology, Microbiology & Immunology
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| Lab |
O'Connell
|
| Street address |
Emma Eccles Jones, Medical Research Building, 15 North Medical Drive East
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112-5650 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA576898 |
| SRA |
SRP225113 |
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