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Series GSE138718 Query DataSets for GSE138718
Status Public on Dec 30, 2019
Title Mitochondrial Pyruvate Carrier 1 (MPC1) promotes peripheral T cell quiescence through metabolic regulation of thymic development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The transporter which uptakes pyruvate into the mitochondria during pyruvate oxidation, called the Mitochondrial Pyruvate Carrier (MPC), was only recently identified. While the MPC has been studied in a variety of cell types, including cancer cells and stem cells, very little is known about the importance of MPC in immune cells. Here, we delete MPC from hematopoietic cells and measure its impact on thymocyte gene expression by 10X Genomics scRNA-seq.
Overall design DAPI negative cells were FACS sorted from homogenized and RBC lysed thymi of 6-10 week old Female MPC1fl/fl (WT) and Vav-Cre MPC1fl/fl (KO). Single cell data.
Contributor(s) Ramstead AG, Wallace JA, O'Connell RM
Citation(s) 32130894
Submission date Oct 10, 2019
Last update date Dec 05, 2022
Contact name Ryan O'Connell
Organization name University of Utah
Department Pathology, Microbiology & Immunology
Lab O'Connell
Street address Emma Eccles Jones, Medical Research Building, 15 North Medical Drive East
City Salt Lake City
State/province UT
ZIP/Postal code 84112-5650
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4116682 Thymocytes, 16036X1 W1
GSM4116683 Thymocytes, 16036X2 W2
GSM4116684 Thymocytes, 16036X3 W3
BioProject PRJNA576898
SRA SRP225113

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Supplementary file Size Download File type/resource
GSE138718_RAW.tar 249.1 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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