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| Status |
Public on Feb 13, 2020 |
| Title |
Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Naïve human pluripotent stem cells (hPSCs) provide a unique experimental platform of cell fate decisions during pre-implantation development, but their lineage potential remains incompletely characterized. As naïve hPSCs share transcriptional and epigenomic signatures with trophoblast cells, it has been proposed that the naïve state may have enhanced predisposition for differentiation along this extraembryonic lineage. Here we examined the trophoblast potential of isogenic naïve and primed hPSCs. We found that naïve hPSCs can directly give rise to human trophoblast stem cells (hTSCs) and undergo further differentiation into both extravillous and syncytiotrophoblast. In contrast, primed hPSCs do not support hTSC derivation, but give rise to non-self-renewing cytotrophoblasts in response to BMP4. Global transcriptome and chromatin accessibility analyses indicate that hTSCs derived from naïve hPSCs acquire features of pre-implantation trophectoderm. The derivation of hTSCs from naïve hPSCs will enable elucidation of early mechanisms that govern normal human trophoblast development and associated pathologies.
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| |
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| Overall design |
Bulk RNA-seq of seven cell types
|
| |
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| Contributor(s) |
Theunissen T, Dong C, Gontarz P, Zhang B |
| Citation(s) |
32048992 |
| Submission date |
Oct 10, 2019 |
| Last update date |
Mar 16, 2020 |
| Contact name |
Bo Zhang |
| E-mail(s) |
bzhang29@wustl.edu
|
| Phone |
3143624757
|
| Organization name |
Washington University School of Medicine
|
| Department |
Developmental Biology
|
| Lab |
Zhang
|
| Street address |
4515 McKinley Research Bldg 03212
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE138762 |
Derivation of trophoblast stem cells from naïve human pluripotent stem cells |
|
| Relations |
| BioProject |
PRJNA576801 |
| SRA |
SRP225050 |
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