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Series GSE138688 Query DataSets for GSE138688
Status Public on Feb 13, 2020
Title Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Naïve human pluripotent stem cells (hPSCs) provide a unique experimental platform of cell fate decisions during pre-implantation development, but their lineage potential remains incompletely characterized. As naïve hPSCs share transcriptional and epigenomic signatures with trophoblast cells, it has been proposed that the naïve state may have enhanced predisposition for differentiation along this extraembryonic lineage. Here we examined the trophoblast potential of isogenic naïve and primed hPSCs. We found that naïve hPSCs can directly give rise to human trophoblast stem cells (hTSCs) and undergo further differentiation into both extravillous and syncytiotrophoblast. In contrast, primed hPSCs do not support hTSC derivation, but give rise to non-self-renewing cytotrophoblasts in response to BMP4. Global transcriptome and chromatin accessibility analyses indicate that hTSCs derived from naïve hPSCs acquire features of pre-implantation trophectoderm. The derivation of hTSCs from naïve hPSCs will enable elucidation of early mechanisms that govern normal human trophoblast development and associated pathologies.
Overall design Bulk RNA-seq of seven cell types
Contributor(s) Theunissen T, Dong C, Gontarz P, Zhang B
Citation(s) 32048992
Submission date Oct 10, 2019
Last update date Mar 16, 2020
Contact name Bo Zhang
Phone 3143624757
Organization name Washington University School of Medicine
Department Developmental Biology
Lab Zhang
Street address 4515 McKinley Research Bldg 03212
City Saint Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (20)
GSM4116149 H9 primed TSC Bulk RNA-seq
GSM4116150 AN primed TSC Bulk RNA-seq
GSM4116151 H9 primed Bulk RNA-seq
This SubSeries is part of SuperSeries:
GSE138762 Derivation of trophoblast stem cells from naïve human pluripotent stem cells
BioProject PRJNA576801
SRA SRP225050

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Supplementary file Size Download File type/resource
GSE138688_counts_table.txt.gz 966.7 Kb (ftp)(http) TXT
GSE138688_fCounts2.txt.gz 481.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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