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Status |
Public on Dec 08, 2019 |
Title |
A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
PRDM14 is a crucial regulator of mouse primordial germ cells (mPGC), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a previous knockdown study indicated that PRDM14 might be dispensable for human germ cell fate. Here, we decided to use inducible degrons for a more rapid and comprehensive PRDM14 depletion. We show that PRDM14 loss results in significantly reduced specification efficiency and an aberrant transcriptome of human PGC-like cells (hPGCLCs) obtained in vitro from human embryonic stem cells (hESCs). Chromatin immunoprecipitation and transcriptomic analyses suggest that PRDM14 cooperates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT signalling and somatic markers. Notably, PRDM14 targets are not conserved between mouse and human, emphasising the divergent molecular mechanisms of PGC specification. The effectiveness of degrons for acute protein depletion is widely applicable in various developmental contexts.
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Overall design |
RNAseq of hESC and hPGCLCs derived from parental line and 2 PRDM14-AID clones with or without Auxin (2 replicates each).
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Contributor(s) |
Sybirna A, Tang WW, Surani MA |
Citation missing |
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Submission date |
Oct 09, 2019 |
Last update date |
Dec 08, 2019 |
Contact name |
Walfred Tang |
E-mail(s) |
walfredtang@gmail.com
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Organization name |
University of Cambridge
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Department |
Gurdon Institute
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Lab |
Azim Surani
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Street address |
Tennis Court Road
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City |
Cambridge |
ZIP/Postal code |
CB2 1QN |
Country |
United Kingdom |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (16)
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This SubSeries is part of SuperSeries: |
GSE138675 |
A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons |
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Relations |
BioProject |
PRJNA576723 |
SRA |
SRP225004 |