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Series GSE138673 Query DataSets for GSE138673
Status Public on Dec 08, 2019
Title A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary PRDM14 is a crucial regulator of mouse primordial germ cells (mPGC), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a previous knockdown study indicated that PRDM14 might be dispensable for human germ cell fate. Here, we decided to use inducible degrons for a more rapid and comprehensive PRDM14 depletion. We show that PRDM14 loss results in significantly reduced specification efficiency and an aberrant transcriptome of human PGC-like cells (hPGCLCs) obtained in vitro from human embryonic stem cells (hESCs). Chromatin immunoprecipitation and transcriptomic analyses suggest that PRDM14 cooperates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT signalling and somatic markers. Notably, PRDM14 targets are not conserved between mouse and human, emphasising the divergent molecular mechanisms of PGC specification. The effectiveness of degrons for acute protein depletion is widely applicable in various developmental contexts.
 
Overall design RNAseq of hESC and hPGCLCs derived from parental line and 2 PRDM14-AID clones with or without Auxin (2 replicates each).
 
Contributor(s) Sybirna A, Tang WW, Surani MA
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Submission date Oct 09, 2019
Last update date Dec 08, 2019
Contact name Walfred Tang
E-mail(s) walfredtang@gmail.com
Organization name University of Cambridge
Department Gurdon Institute
Lab Azim Surani
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (16)
GSM4115948 ESC_4i_Par_IAA_r1
GSM4115949 ESC_4i_Par_IAA_r2
GSM4115950 ESC_4i_Par_noIAA_r1
This SubSeries is part of SuperSeries:
GSE138675 A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Relations
BioProject PRJNA576723
SRA SRP225004

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138673_DEseq2_log2_normalized_count.txt.gz 2.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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